Biopolymers and cell. 2006; 22 (1): 38 - 48

Overexpression of genes at different stages of astrocytic glioma development

V. V. Dmytrenko, O. I. Boyko, C. O. Shostak, O. E. Symyrenko, T. V. Bukreyeva, V. D. Rozumenko, T. A. Malysheva, M. I. Shamaev, Y. P. Zozulya, V. M. Kavsan

Public database of Serial Analysis of Gene Expression (SAGE) was used for identification of potential human astrocytic glioma molecular markers. The comparison of nine glioblastoma SAGE-libraries, eleven anaplastic astrocytoma SAGE-libraries, eight diffusive astrocytoma SAGE-libraries, and five human normal brain SAGE-libraries revealed 57 genes with more than 5-fold increase of expression in astrocytic gliomas (P≤0.05) comparing to the human normal brain. Besides the genes expression changes which occur at the early stage of astrocytomas formations and are revealed also during the subsequent stages of progression, some changes are characteristic only of highly malignant stages of tumor development while they are absent in the tumors of low stage of malignancy. The analysis of revealed genes expression can be used for glial tumor molecular classification, diagnosis, prognostic evaluation and determination of potential targets for anticancer therapy.

Key words: SAGE, differential expression, astrocytic glioma, molecular markers.