Biopolymers and cell. 2006; 22 (2): 143 - 148

Copolymeric hydrogel membranes for immobilization and cultivation of human stem cells

O. O. Kosenko, L. L. Lukash, U. M. Samchenko, T. A. Ruban, Z. R. Ulberg, S. I. Lukash

A number of hydrogel membranes for stem cells cultivation has been synthesized on the basis of acrylamide, acrylonitrile, and acrylic acid by the method of radical copolymerization. The comparison has shown that the optimal parameters, determined by the immobilization of human mesenchymal stem cells, are inherent to nonionic copolymer hydrogels on acrylamide and acrylonitrile bases. The use of hydrogel with considerable acrylic acid content is limited by pH decrease in cultivation medium, which is incompatible with life activity maintenance of the cells under investigation.

Key words: hydrogel, stem cells, immobilization, cultivation medium.