Biopolymers and cell. 2006; 22 (4): 299 - 306

Cytogenetic analysis of the spontaneously immortalized mouse cell line G1

A. P. Iatsyshyna, O. V. Pidpala, T. P. Kochubey, L. L. Lukash

Cytogenetic analysis of the spontaneously immortalized mouse cell line G1 has been carried out on passages 15, 24, 53, 68, 104 and 140 of in vitro cultivation. G1 cells were revealed to have quantitative chromosome changes, different chromosomal aberrations, and a particularly high percentage of Robertsonian translocations. The heterogeneity of cell population was observed to diminish during long-term cell cultivation. The karyotypic evolution of cells lasted not less than 140 passages on the stage of formation.

Key words: karyotypic evolution; chromosome aberration; Robertsonian translocation.