Biopolymers and cell. 2006; 22 (5): 350 - 354

The Sources of Heterogeneity of Embryo Cell Populations of Mouse Line BALB/c Cultured In Vitro

T.T. Glazko, A.P. Iatsyshyna, O.V. Pidpala, I.V. Vavilina, L.L. Lukash

Detailed chromosomal analysis of embryo mouse cell line G1 on the 15th passage of in vitro cultivation has been performed. Possible mechanisms of heterogeneity of the investigated cell line are discussed. Spontaneous chromosome G-staining has been observed in some metaphase plates. Premature disjunction of sister centromeres has been found, in comparison with other regions of sister chromatids. The distribution of rearranged chromosomes (including Robertsonian translocations), the presence of autonomous chromosome clusters, associated in pericentromeric regions, as well as despiralization of pericentromeric heterochromatin blocks designate the similarity of metaphase chromosomes of a novel mouse G1 cell line to embryonic stem cells and embryonic germ cells of rodents.

Key words: mouse cell line, cell population heterogeneity, chromosomal aberrations.