Biopolymers and cell. 2006; 22 (5): 355 - 362

Antibiotic resistance changes in strains of bacteria and yeast-like fungi following their growth in established cell lines of human and animal origin

S. L. Rybalko, Ye. V. Pokas, V. A. Dieyev, T. M. Liaskovski, T. M. Furzikova, S. T. Diadiun, N. V. Ivanskaya, N. I. Nastoyashcha, P. P. Verkhatsky, S. T.Salkov

The phenomenon of the susceptibility and reversion of resistance to antibiotics in strains of bacteria and yeast-like molds has been identified upon the interaction of bacteria and molds with transplantable human and animal cell lines depending on the cultivation conditions. The ability of susceptibility reversion of bacteria and yeast-like fungi to some antibiotics is not stable, it varies with the passages. This phenomenon may be a reason of the poor efficacy of antibiotic treatment when the susceptibility of bacteria to antibiotics is assayed by the usual technique. The authors propose to modify the antibiotics-susceptibility assay currently used in clinics by co-culture of the isolated bacteria and fungi with the transplantable cell lines or human leukocytes.

Key words: antibiotics, cells, enterobacteria, resistance.