Biopolymers and cell. 2007; 23 (2): 137 - 142

Synthesis of 3'- and 3',5'-modified oligonucleotides on functionalized silica Silochrom-2

I. Ya. Dubey, L. V. Dubey, D. M. Fedoryak

The preparation of 3'-mono- and 3', 5'-bis-labeled oligonucleotides on a new fluorescein-functionalized polymer support based on Silochrom-2 microspherical silica is described. Oligonucleotide (dT)₁₅ containing a dye residue at 3'-end was synthesized on this support by solid-phase phosphoramidite method. Post-synthetic 5'-modification of 3'-conjugate allowed obtaining double dye-labeled oligomer.

Keywords: oligonucleotide conjugates, fluorescent labels, solid-phase synthesis, functionalization.