Biopolymers and cell. 2007; 23 (6): 495 - 500

Development and characterization of monospecific anti-Sgt1 antibodies

L. N. Kapustian, O. T. Rozhko, V. I. Bobyk, I. V. Kroupskaya, L. L. Sidorik

The method of development and purification of monospecific polyclonal antibodies against Sgt1 was described and their characteristics were presented. Protein Sgt1 plays an important role in the regulation of cell cycle, Hsp90-related proteasome degradation of proteins and pro/anti-apoptotic signaling along with other molecular chaperons and co-chaperons. The further investigation of Sgt1 role and its functioning with other members of Hsp90-chaperons family is important for elucidation of the cell signaling regulation, which is significant for cardio-vascular pathologies progression, particularly, for dilated cardiomyopathy.

Keywords: monospecific antibodies, Sgt1, chaperons.