Biopolymers and cell. 2008; 24 (5): 399 - 405

Choice of conditions of human bone marrow stromal cells seeding into polymer macroporus sponges

Yu. A. Petrenko, N. A. Volkova, E. P. Zhulikova, L. G. Damshkaln, V. I. Lozinsky, A. Yu. Petrenko

Different conditions of seeding human bone marrow stromal cells (SC) into polymer sponges based on macroporous agarose cryogel (MACS) were investigated. Three methods of cell seeding to MACS were used: co-incubation with permanent stirring, intensive shaking and creation of negative pressure. The results obtained show considerable potential of macroporous agarose cryogel sponges as a three-dimensional carrier for cultivating stromal cells at different methods of cell seeding.

Keywords: tissue engineering, bone marrow stromal cells, macroporous sponges, agarose cryogel, seeding, Alamar Blue.