Biopolymers and cell. 2008; 24 (6): 487 - 493

Somaclonal variability of Ungernia victoris: the necessity of comprehensive genetic analysis

O. M. Bublyk, I. O. Andreev, E. V. Spiridonova, V. A. Kunakh

An analysis of five 10-year-old U. victoris cell lines of common origin revealed mixoploidy, significant level of cell polyploidization, quantitative and qualitative changes in individual RAPD-fragments. No regularity was observed in the lines clustering depending on the nutrient medium phytohormone composition or genealogy of the lines. The cytogenetic, molecular and genetic approaches are recommended to ensure reliable determination of somaclonal changes, because the variations in ploidy levels do not always result in the RAPD-polymorphism

Keywords: somaclonal variation, Ungernia victoris, RAPD-analysis, cytogenetic analysis.