Biopolymers and cell. 2009; 25 (1): 56 - 61

Photoactive immunoconjugate of hematoporphyrin with antibodies to vascular endothelial growth factor: preparation, physicochemical and biological properties

I. O. Lisniak, N. L. Novichenko, A. A. Mamchur, M. F. Gamaliya

R.E.Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine 45, Vasylkivska, Kyiv, Ukraine, 03022

Preparation of photoimmunoconjugates on the basis of hematoporphyrin and antibodies to the vascular endothelial growth factor (VEGF) is described. The obtained conjugate preserved physicochemical and biological properties of both protein component and porphyrin part – hematoporphyrin. It was shown that accumulation of the photoimmunoconjugate with VEGF antibodies in chicken embryo membranes was 2,5 times larger at the period of the neovascularization than at the stabilization.

Keywords: photodynamic therapy, angiogenesis, conjugates, hematoporphyrin, VEGF.