Biopolymers and cell. 2009; 25 (2): 115 - 119
Detection of Methylobacterium radiotolerans IMBG290 in potato plants by in situ hybridization
O. V. Podolich, L. P. Ovcharenko1, N. O. Kozyrovska1, A. M. Pirttilà2
Institute of Agroecology of UAAS 12 Metrologichna str., Kyiv,
Ukraine, 03680
1Institute of Molecular Biology and Genetics of National Academy of
Sciences of Ukraine 150 Acad. Zabolotnoho str., Kyiv, Ukraine, 03680
2Department of Biology University of Oulu Linnanmaa, PO Box 3000,
FIN-90014 Oulu, Finland
A new bacterial strain of pink-pigmented facultative methylotroph (M. radiotolerans IMBG290) which was previously isolated from in vitro grown potato plantlets after their inoculation with Pseudomonas fluorescens IMBG163 was detected in tissues by in situ hybridization method (ISH/FISH). The presence of Methylobacterium rRNA was observed in leaves and stems of potato plantlets, whereas no signal was detected in potato roots. The signal was less abundant in the untreated plants than in the plantlets infected with M. radiotolerans IMBG290.
Keywords: in situ hybridization, Methylobacterium radiotolerans IMBG290, potato plantlets.