Biopolymers and cell. 2009; 25 (4): 279 - 289
The procedure of ethanol determination in wine by enzyme amperometric biosensor
T. B. Goriushkina1,2, A. P. Orlova1,2, G. M. Veryk3, A. P. Soldatkin1, S. V. Dzyadevych1
1Institute of molecular biology and genetics NAS of Ukraine
150, Zabolotnogo Str, Kyiv Ukraine, 03680
Aim. Development of the procedure of ethanol determination in wine by an enzyme amperometric biosensor. Methods.
The amperometric biosensor method of ethanol analysis has been used in this work. Results. The paper presents comparative analysis
of two methods of alcohol oxidase (AO) immobilization for development of amperometric biosensor for ethanol determination in wine.
The method of AO immobilization in glutaraldehyde vapour was chosen as optimal for this purpose. The selectivity, operational and
storage stability, and pH-optimum for operation of the created biosensor were determined. The procedure of ethanol determination
in wine by amperometric biosensor on the basis of platinum printed electrode SensLab and AO was optimized.
The analysis of ethanol concentration in wine and must samples was carried out using the developed high-stable
biosensor. A good correlation between the data obtained by the biosensor and densitometry methods was shown.
Conclusion. The proposed method of ethanol analysis could be used in wine production.
Keywords: amperometric biosensor, alcohol oxidase, ethanol, wine, must.
2National Taras Shevchenko University of Kyiv
64, Volodymyrska Str, Kyiv, Ukraine, 01033
3Institute for Vine and Wine "Magarach", 31 Kirov St., Yalta, Crimea, Ukraine, 98600