Biopolymers and cell. 2010; 26 (1): 23 - 28
The effect of lipopolysaccharide of Pseudomonas syringae pv. atrofaciens 9417 on mutagenicity in pro- and eukaryotic systems
Yu. M. Bogdan, L. M. Butsenko, L. A. Pasichnyk, R. I. Gvozdyak
Institute of microbiology and virology 154, Zabolotnogo Str, Kyiv Ukraine, 03680
Aim. To study the effect of lipopolysaccharide (LPS) of Pseudomonas syringae pv. atrofaciens on spontaneous and induced mutations in pro- and eukaryotic test-systems. Methods. Mutagenic and antimutagenic properties of LPS were studied in Allium cepa-test and Ames test. Results. LPS does not influence the spontaneous mutations of Salmonella typhimurium and decreases the level of mutations induced by potassium dichromate and N-methyl-N'-nitro-N'-nitrosoguanidine. LPS reduces a mitotic index at concentrations of 10.0 and 5.0 mg/ml and increases the number of chromosomes’ fragments in cells of A. cepa root apical meristem at concentrations of 5.0 and 2.5 mg/ml. Conclusion. Different effect of LPS on mutagenesis in pro- and eukaryotic cells has been established. LPS revealed mutagenic properties in A. cepa-test and antimutagenic properties in Ames test.
Keywords: mutations, chromosome aberrations, LPS.