Biopolymers and cell. 2010; 26 (2): 121 - 127
Oxidative stress, advanced glycation end products and residual renal function in the rat model of unilateral ureteral obstruction: effects of phlogenzym and losartan
K. Sebekova, Jr., P. Blazicek1, D. Syrova2, S. Galbavy3, R. Schinzel4, A. Heidland4, K. Sebekova5
St. Elisabeth and Barbara Hospital 5, Mauerstrabe, Halle/Saale, Germany, 06110
1Alpha Medical 49, Vlcie hrdlo, Slovakia, 81207
2Children Hospital 1, Limbova, Bratislava, Slovakia, 83340
3St. Elisabeth University of Health and Social Sciences 1, Namestie, maja, Bratislava, Slovakia, 81100
4University of Wurzburg 6, Oberdurrbacher Strabe, Wurzburg, Germany, 97080
5Slovak Medical University 12, Limbova, Bratislava, Slovakia, 83303
Aim. Oxidative stress plays a role in the pathogenesis of ureteral obstruction. Methods. We studied parameters of oxidative status, levels of advanced glycation end products (AGEs), and contralateral (CL) kidney function in the rat model of unilateral ureteral obstruction (UUO). The effect of Phlogenzym (12 mg/day orally); losartan (20 mg/l in drinking water), and their combination was studied. Results. In placebo-administered UUO rats AGEs and malondialdehyde levels were higher than in the sham operated controls. Function of the CL kidney was slightly impaired, its collagen content and protein/deoxyribonucleic acid ratio (P/DNA) in the glomeruli increased. All treatments prevented the rise in collagen content, P/DNA ratio, and improved CL kidney function. Phlogenzym ameliorated lipid peroxidation and AGE levels. Conclusions. In the model of UUO systemically increased oxidative stress may play a role in development of tubulointerstitial fibrosis and in the functional impairment of the CL kidney. Suppression of the oxidative stress and blockade of angiotensin-1 receptors might mitigate the progression of obstructive uropathy.
Keywords: ureteral obstruction, advanced glycation end products, oxidative stress, malondialdehyde, collagen.