Biopolymers and Cell. 2010; 26 (5): 373-377
Identification of autoantibodies to tyrosil-tRNA synthetase in heart disfunctions
Iu. Iu. Kondratiuk1, 2, L. L.Sidorik1, V. I. Bobyk1, D. V. Ryabenko3, A. I. Kornelyuk1
1Institute of Molecular Biology and Genetics, NAS of Ukraine; 150, Zabolotnoho Str., Kyiv, Ukraine, 03680
2Taras Shevchenko National University of Kyiv; 64, Volodymyrska Str., Kyiv, Ukraine, 01033
3National Scientific Centre «M. Strazhesko Institute of Cardiology» AMS of Ukraine; 5, Narodnoho Opolchennya Str., Kyiv, Ukraine, 03151
Aim. To investigate the levels of specific autoantibodies against tyrosyl-tRNA synthetase and its individual modules in the blood serum of people with heart failure caused by dilated cardiomyopathy, myocarditis and ischemic heart disease compared with healthy donors. Methods. Recombinant proteins were obtained using bacterial strains transformed with appropriate plasmid vectors and were purified by chromatography on Ni-NTA-agarose. The levels of specific autoantibodies were investigated by ELISA. Results. The increased levels of autoantibodies specific to tyrosyl-tRNA synthetase, its N-terminal catalytic module and non-catalytic C-module, were found in the blood serum of patients, compared with healthy donors. Conclusions. The results obtained demonstrate the possible role of tyrosyl-tRNA synthetase in adaptive changes of the myocardium in response to stress factors.
Keywords: autoantibodies, tyrosyl-tRNA synthetase, heart failure.