Biopolymers and Cell. 2012; 28(2): 129-133

 

MICE LACKING PITUITARY TUMOR TRANSFORMING GENE SHOW ELEVATED EXPOSURE OF DGALNAC CARBOHYDRATE DETERMINANTS

 

Varyvoda O. Yu., 1Filyak Yu. Z., Lutsyk A. D., 1Stoika R. S.


Danylo Halytsky Lviv National Medical University
69, Pekarska str., Lviv, Ukraine, 79010

1Institute of Cell Biology, NAS of Ukraine
14/16, Drahomanov Str., Lviv, Ukraine, 79005

Aim. To investigate the influence of pituitary tumor transforming gene (pttg-1) knockout on glycome of parenchimal organs by means of lectin histochemistry. Methods. DGalNAc, DGlcNAc, NeuNAc carbohydrate determinants were labelled with soybean agglutinin (SBA) and wheat germ agglutinin (WGA), conjugated to peroxidase, with subsequent visualization of the lectin-binding sites with diaminobenzidine. The testes and kidneys of murine strain BL6/C57 with the pttg-1 gene knockout (PTTG-KO) were compared to the wild type (PTTG-WT) animals, both groups 1 month of age. Results. Knockout of the pttg-1 gene was accompanied by enhanced exposure of the DGalNAc sugar residues within the Golgi complex of secondary spermatocytes, in a brush border of renal tubules and on the lumenal surface of collecting ducts. Conclusions. This study suggests that knockout of the pttg-1 gene may lead to the changes in carbohydrate processing in mammalian organism.

 


Keywords: knockout of pttg-1 gene, glycoconjugate processing, lectin histochemistry

 

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