Biopolymers and Cell. 2012; 28(2): 149-155
ALLELES OF PPD-D1 GENE IN THE COLLECTION OF AEGILOPS TAUSCHII ACCESSIONS AND BREAD WHEAT VARIETIES
1Chebotar G. O., 1, 2Chebotar S. V., 2Babenko D. O., 3Motsnyy I. I., 4Scherban A. B., 1Sivolap Yu. M.
1South Plant Biotechnology Center, NAAS of Ukraine
3, Ovidiopolska doroga, Odesa, Ukraine, 65036
2Odesa I. I. Mechnikov National University
2, Dvoryanska Str., Odesa, Ukraine, 65082
3Plant Breeding and Genetics Institute - National Center of Seed and
Cultivar Investigation
3, Ovidiopolska doroga, Odesa, Ukraine, 65036
4The Institute of Cytology and Genetics, the Siberian Branch of the
Russian Academy of Sciences
10, Prosp. Akad. Lavrentyeva, Novosibirsk, Russian Federation, 630090
Light period significantly influences on the growth and development of plants. One of the major genes of photoperiod sensitivity is Ppd-D1, located on the chromosome 2D. The aim of the work was to determine the alleles and molecular structure of Ppd-D1 gene in samples from the collection of Ae. tauschii accessions, which have different flowering periods, and in 29 Ukrainian wheat varieties. Methods. We used methods of allele-specific PCR with primers to the Ppd-D1 gene, sequencing and Blast-analysis. Results. The collection of Ae. tauschii accessions and several varieties of winter and spring wheat was studied. The molecular structure of the allelic variants (414, 429 and 453 b. p.) of Ppd-D1b gene was determined in the collection of Aegilops. tauschii accessions. Conclusions. The Ppd-D1a allele was present in all studied varieties of winter wheat. 60 % of spring wheat is characterized by Ppd-D1b allele (size of amplification products 414 b. p.). Blast-analysis of the sequence data banks on the basis of the reference sequence of sample k-1322 from the collection of Ae. tauschii accessions has shown a high homology (80 to 100 %) between the nucleotide sequences of PRR genes, that characterize the A and D genomes of representatives of the genera Triticum and Aegilops. |
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