Biopolym. Cell. 1993; 9(5):19-25.
Structure and Function of Biopolymers
Influence of antiarrhythmic and nootropic drugs on model and biological membranes
1, 2Beregovaja E. G., 1Goryshko A. G., 1Checkman I. S., 1Dunayev V. V., 1Sachenko L. G.
  1. Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine
    14, Eugene Pottier Str., Kyiv, Ukraine, 03057
  2. Zaporizhzhya Medical Institute
    Mayakovsky avenue 26, Zaporozhye, Ukraine, 69035


Membranotropic action of antiarrythmic and nootropic drugs (anaprilin, etmozine, carnithine, piracetam, phenhisole, ksantiverine, phenoxanone, compounds E and VS) was studied on lyposomes from lecithine, cardiolypine and synaptosomal membranes of rabbit brain by means of spectrofotometry and spectrofluorometry using fluorescent probes of pyrene. The ability of these compounds to form complexes with lipid bilayer, increase of its motility, was shown. The studied physiologically active compounds decrease calcium-binding properties of synaptosomal membranes and of bilayer from cardiolypine.


[1] Porotikov VI, Lazarev AV, Glezer GA. Current concepts of the ionic mechanisms of cardiac arrhythmias and the possible mechanisms of action of cardiac anti-arrhythmia agents. Usp Fiziol Nauk. 1984;15(3):42-63.
[2] Vititnova MB, Kacharova VG, Saks VA Stabilization membrane phospholipids cardiocytes - a new approach to the prevention of cardiac arrhythmias in the acute phase of myocardial infarction. Proc. of reports. VI Proc. Congress of Pharmacology. Tashkent, 1988:71.
[3] Ovchinnikov IV. Effect of obzidan and Novodrin on the phospholipid composition of the myocardium. Farmakol Toksikol. 1983;46(5):37-40.
[4] Chekman JS Biochemical and physico-chemical mechanisms of action of drugs. Proc. of reports. VI Proc. Congress of Pharmacology. Tashkent, 1988: 408.
[5] Chatelain P, Brotelle R, Laruel R. Decrease in lipid mobility in rat erythrocyte membrane after amiodarone chronic treatment. Biochem Pharmacol. 1987;36(9):1564-5.
[6] Erdreich A, Rahamimoff H. The possible involvement of the phospholipid phase of membranes in mediating the effects of verapamil on Ca2+ transport. Biochem Pharmacol. 1987;36(11):1775-80.
[7] Vladimirov YuA., Dobretsov GE. Fluorescent Probes in the study of biological membranes. Moscow: Nauka, 1980. 320 p.
[8] Ferreira J, Chatelain P, Caspers J, Ruysschaert JM. Ionization state of amiodarone mediates its mode of interaction with lipid bilayers. Biochem Pharmacol. 1987;36(24):4245-50.
[9] Stets VR, Beregovaia EG, Dunaev VV, Storozhuk BG, Favoritov VN, Stoliarchuk AA, Mazur AI. The anti-arrhythmia activity of quinazopyrine. Farmakol Toksikol. 1990;53(3):15-7.
[10] SteTS VP. Pharmacological study of new derivatives of azoles and azines: Auto-Ref. dis .... Dr. med. nauk. Kazan, 1988. 42 p.
[11] Dounaev VV Tishkin VS Coast EG Pharmacological Study of antiarrhythmic properties of the drug carnitine chloride injection. Rhythm and conduction disturbances. Tez. of reports. Tez. dokl. Plenuma pravleniya ukr. nauch. kardiol. ob-va-Zaporozh'ye, 1990:31.
[12] Shavaryan VV Yagupolsky LM Features pharmacodynamics of some new derivatives of pyridine and pyrimidine. Tez. of reports. VI Congress of Ukrainian pharmacologists SSSR. Kharkov, 1990: 432-433.
[13] Samvelian VM, Malakian MG, Badzhinian SA. The antiarrhythmic action of piracetam. Farmakol Toksikol. 1990;53(6):22-3.
[14] Mashkovskiy MD. Drugs. M: Meditsina. 1984. vol 1:400-9.
[15] Lebed' OI, Stefanov AV, Primak RG. Effect of the conditions of ultrasonic treatment on the characteristics of forming liposomes. Ukr Biokhim Zh. 1989;61(3):96-101.
[16] Lishko VK, Terletskaya LT, Gegelashvili GK. Interaction isolated synaptic vesicles with membranes as a model sinatstosom beirosection. Biol membrane. 1986; 3(2):191-6.
[17] Practicum in biochemistry. Ed. NP Lishkovoy, E. E. Severinich. M. : MGU, 1979: 90.
[18] Kendrick NC, Ratzlaff RW, Blaustein MP. Arsenazo III as an indicator for ionized calcium in physiological salt solutions: its use for determination of the CaATP dissociation constant. Anal Biochem. 1977;83(2):433-50.
[19] Sverdlova OV. Electronic spectra in organic chemistry. L: Khimiya, 1985. 248 p.
[20] Dobretsov GE. Fluorescent probes in the study of cell membranes and lipoproteins. Moscow: Nauka, 1989. 277 p.
[21] Bauer PJ. Affinity and stoichiometry of calcium binding by arsenazo III. Anal Biochem. 1981;110(1):61-72.
[22] Sim E. Membrane biochemistry. London ; Chapman and Hall, 1982. 80 p.