Biopolym. Cell. 2006; 22(6):452-457.
Genome and Its Regulation
Polymorphism of genes of methylenetetrahydrofolate reductase, glutathione S-transferase P1 and M1, cytochrome P450 1A1 and glutathione S-transferase activity in human placenta
1, 2Martresyuk O. P., 1Teplyuk N. M., 1Sazonova L. Y., 3Mislanova C., 1Obolenskaya M. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033
  3. Institute of Preventive and Clinical Medicine
    Limbova 14, Bratislava, Slovak Republic, 83301

Abstract

For the first time the distribution frequency of 677 methyle­netetrahydrofolate reductase (MTHFR) genotypes was detected in a limited part of Ukrainian population. Cytosolic activity in human placenta samples with different allelic gene variants of MTHFR in combinations with polymorphic genes of glutathione S-transferase PI, glutathione S-transferase Ml, and cytochrome P450 1A1 was analyzed. The substitution of C677→T677 in at least one allele of MTHFR gene is accompanied by the tendency of GST activity to increase. The tendency remains irrespectively of other allelic gene variants combination. The possible relation between the MTHFR allelic variants and cytosolic glutathione S-transferase activity is discussed.
Keywords: polymorphism, methylenetetrahydrofolate reductase, glutathione S-transferase P1 and M1, human placenta, glutathione S-transferase activity.

References

[1] Matthews RG. Methylenetetrahydrofolate reductase: a common human polymorphism and its biochemical implications. Chem Rec. 2002;2(1):4-12.
[2] van der Put NM, Gabre?ls F, Stevens EM, Smeitink JA, Trijbels FJ, Eskes TK, van den Heuvel LP, Blom HJ. A second common mutation in the methylenetetrahydrofolate reductase gene: an additional risk factor for neural-tube defects? Am J Hum Genet. 1998;62(5):1044-51.
[3] Heijmans BT, Boer JM, Suchiman HE, Cornelisse CJ, Westendorp RG, Kromhout D, Feskens EJ, Slagboom PE. A common variant of the methylenetetrahydrofolate reductase gene (1p36) is associated with an increased risk of cancer. Cancer Res. 2003;63(6):1249-53.
[4] Mills JL, McPartlin JM, Kirke PN, Lee YJ, Conley MR, Weir DG, Scott JM. Homocysteine metabolism in pregnancies complicated by neural-tube defects. Lancet. 1995;345(8943):149-51.
[5] Teplyuk NM, Lebedeva LM, Kolomiets LI, Butkevich DM, Khoronzhi MR, Puar'e MS, Obolenskaia MIu. [Pheno-and genotyping of a placental detoxication system in ecologically unfavorable regions of Ukraine]. Ukr Biokhim Zh. 2001;73(3):126-34.
[6] Obolens'ka MIu, ChaÄ­kovs'ka TL, Lebedieva LM, Teplyuk NM, Kolomiiets' LI, Ivans'ka NV, Didenko LV, Nekrich VV, Burlak HF. [Detoxicating function of the placenta of childbearing women from ecologically unfavorable regions of the Ukraine]. Ukr Biokhim Zh. 1998;70(2):89-97.
[7] Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988;16(3):1215.
[8] Van Amerongen G, Mathonnet F, Boucly C, Mathieu B, Vinatier I, Peltier JY, Catherine N, Collet C, de Mazancourt P. An improved method for the detection of the thermolabile variant of methylenetetrahydrofolate reductase. Clin Chem. 1998;44(5):1045-7.
[9] Pabst MJ, Habig WH, Jakoby WB. Glutathione S-transferase A. A novel kinetic mechanism in which the major reaction pathway depends on substrate concentration. J Biol Chem. 1974;249(22):7140-7.
[10] McAndrew PE, Brandt JT, Pearl DK, Prior TW. The incidence of the gene for thermolabile methylene tetrahydrofolate reductase in African Americans. Thromb Res. 1996;83(2):195-8.
[11] Gasparovic J, Raslov? K, Basistov? Z, Zacharov? M, Ws?lov? L, Avdicov? M, Blaz?cek P, Lietava J, Siv?kov? D. Effect of C677T methylenetetrahydrofolate reductase gene polymorphism on plasma homocysteine levels in ethnic groups. Physiol Res. 2004;53(2):215-8.
[12] Teplyuk NM, Samoylenko AA, Lebedeva LM, Sazonova LYu, Shcherbina MS, Perepelyuk MM, Obolenska MYu. Detoxication function of placenta and its peculiarities due to P4501A1, GSTP1 and Ml genotypes. NaUKMA Academic Records. 2002; 20(2): 445-7.
[13] Teplyuk N, Sazonova L, Melnyk A, Divi R, Puarie M, Obolenska M. Glutathionetransferase activity and DNA-adducts in the human placenta in adverse environmental conditions. NaUKMA Academic Records. 2003; 22(3):379-82.
[14] Obolenskaya M, Teplyuk N, Prima V, Malko M, Bondarenko E, Didenko L, Vit V, Divi R, Poirier M, Pasanen M. Glutathionetransferase activity and PAH-DNA adducts in human placenta as a risk factor for new-born in radioactively contaminated regions. Int J Radiat Med. 2004; 6: 154-66.
[15] Crofts F, Cosma GN, Currie D, Taioli E, Toniolo P, Garte SJ. A novel CYP1A1 gene polymorphism in African-Americans. Carcinogenesis. 1993;14(9):1729–31.
[16] Zimniak P, Nanduri B, Piku?a S, Bandorowicz-Piku?a J, Singhal SS, Srivastava SK, Awasthi S, Awasthi YC. Naturally occurring human glutathione S-transferase GSTP1-1 isoforms with isoleucine and valine in position 104 differ in enzymic properties. Eur J Biochem. 1994;224(3):893-9.