Biopolym. Cell. 2007; 23(2):137-142.
Биоорганическая химия
Синтез 3'- и 3', 5'-модифицированных олигонуклеотидов на функционализированном силикагеле «Силохром-2»
1Дубей И. Я., 1Дубей Л. В., 2Федоряк Д. М.
  1. Институт молекулярной биологии и генетики НАН Украины
    ул. Академика Заболотного, 150, Киев, Украина, 03680
  2. Институт биоорганической химии и нефтехимии НАН Украины
    ул. Мурманская, 1, Киев, Украина, 02094


Описано получение З'-моно- и 3',5'-бис-меченных олигонуклео­тидов на новом полимерном носителе с использованием микосферического силикагеля «Силохром2», функционализирован­ном флуоресиеином. На этом полимере твердофазным фосфитамидным методом синтезирован олигонуклеотид (dT)15, содержащий остаток красителя на 3'-конце. Постсинтетиче­ская 5'-модификация З'-конъюгата позволила получить олигомер, меченный двумя молекулами флуоресцеина. В будущем это будет использовано для синтеза флуоресцентных зондов для детекции нуклеиновых кислот.
Keywords: олигонуклеотидные конъюгаты, флуоресцентные метки, твердофазный синтез, функционализация


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