Biopolym. Cell. 2008; 24(5):377-384.
Структура и функции биополимеров
Получение рекомбинантного дефензина 1 сосны обыкновенной и его антифунгальная активность
1Ковалева В. А., 1Гут Р. Т., 2Гут И. Т.
  1. Национальный лесотехнический университет Украины
    ул. Генерала Чупринки, 103, Львов, Украина, 79057
  2. Университетский колледж Лондона
    ул. Гавер, Лондон, WC1E 6BT, Великобритания

Abstract

Ранее нами очищен эндогенный дефензин из проростков сосны обыкновенной, а также клонирована кДНК, кодирующая дефензин 1 (PsDef1, Pinus sylvestris дефензин 1). Фрагмент кДНК дефензина 1, кодирующий зрелую форму этого белка, клонирован в вектор pET42а(+) и индуцирована его экспрессия в бактериальной системе Escherichia coli. Оптимизированы условия продукции рекомбинантного белка PsDef1, слитого с глутатион-S-трансферазой, в растворимой форме. После аффинной очистки этого белка на глутатион-сефарозе и протеолиза фактором Ха получен биологически активный препарат рекомбинантного PsDef1, антифунгальная активность которого соизмерима з таковой эндогенного дефензина 1 сосны обыкновенной.
Keywords: рекомбинантный дефензин PsDef1, экспрессия, аффинная очистка, антифунгальная активность

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