Biopolym. Cell. 1987; 3(2):88-95.
Генно-инженерная биотехнология
Амплификация генов дигидрофолатредуктазы и их перенос в животные клетки
1Кузнецова Н. Н., 1Мухамедханова Ф. С., 1Нуриджанянц С. С., 1Абдукаримов А. А., 1Садыков А. С.
  1. Институт биоорганической химии АН Узбекской ССР
    Ташкент, СССР

Abstract

Описана новая сублиния клеток китайского хомячка BFFR3, выведенная на основе фибробластоподобной клеточной линии Blld-ii-FAF28 (клон 431), резистентная к 132 мкг/мл метотрексата. Хромосомный анализ резистентных клеток позволил обнаружить специфические изменения кариотипа в виде гомогенно окрашенных областей на 2-й и 5-й хромосомах, а также изменение модального класса резистентных клеток. Используя ДНК резистентных клеток, осуществлен перенос лекарственной устойчивости в чувствительные FStk–клетки.

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