Biopolym. Cell. 2015; 31(4):243-248.
Обзоры
CRISPR/Cas9 технология для целевого редактирования генома
1, 3Ломов Н. А., 1, 3Борунова В. В., 1, 2, 3Рубцов М. А.
  1. Московский государственный университет имени М. В. Ломоносова
    Ленинские горы, 1/12, Москва, Российская Федерация, 119991
  2. Первый Московский Государственный Медицинский Университет им. И. М. Сеченова Минздрава России
    ул. Трубецкая, д. 8, стр. 2, Москва, Российская Федерация, 119991
  3. LIA 1066 Объединенная французско-российская лаборатория по исследованию рака
    Вильжюиф, Франция-Москва, Российская Федерация

Abstract

CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) – последовательности в геноме прокариот, которые состоят из коротких повторов, перемежающихся уникальными последовательностями. Это система бактериальной защиты от вирусной ДНК. Молекулярные компоненты данной системы с 2013 года используются как инструмент редактирования эукариотического генома, хотя данная технология и имеет некоторые ограничения и недостатки. В данном обзоре мы затронем историю применения системы CRISPR/Cas9 и обсудим возможности, которые данная технология предоставляет для исследования и лечения различных заболеваний.
Keywords: CRISPR/Cas9, таргетинг генома, редактирование геномаg, персонализированая терапия, хромосоные транслокации, репарация ДНК

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