Biopolym. Cell. 2020; 36(5):358-370.
Биоорганическая химия
Флуоресцентные конъюгаты D-глюкозамина с 3-тиазолилкумаринами: синтез, характеризация и потенциальное использование как реагентов для визуализации клеток
1Кузив Я. Б., 1Новосильная А. В., 1Дубей И. Я.
  1. Институт молекулярной биологии и генетики НАН Украины
    ул. Академика Заболотного, 150, Киев, Украина, 03143

Abstract

Цель. Синтез и исследование конъюгатов глюкозамин-кумарин как возможных реагентов для флуоресцентной визуализации клеток. Методы. Органический синтез, ЯМР, оптическая спектроскопия, конфокальная микроскопия. Результаты. Конъюгаты D-глюкозамина с 7-замамещенными 3-тиазолилкумаринами получены с выходами 55-82% реакцией NHS-активированных эфиров карбокси-модифицированных красителей с аминогруппой сахара. При помощи ЯМР определена аномерная конфигурация продуктов. Исследованы их спектральные свойства в метаноле и фосфатном буфере. Предварительно оценена возможность применения конъюгатов для окрашивания клеток. Выводы. Тиазолилкумарин-меченые глюкозамины с яркой голубой эмиссией – перспективные реагенты для визуализации клеток.
Keywords: кумарины, глюкозамин, конъюгаты, флуоресценция, конфокальная микроскопия

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