Biopolym. Cell. 1993; 9(2):19-23.
Viruses and Cell
Nonradioactive hybridization test of Plasmodium falciparum infection of humans
1Adarichev V. A., 1Dymshits G. M., 2Mishina E. S., 1Salganik R. I., 2Sochanenkova T. L.
  1. Institute of Cytology and Genetics, Siberian Branch of the RAS
    10, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090
  2. E .I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine
    20, M. Pirogov Str., Moscow, Russian Federation, 119435

Abstract

Nonradioactive molecular hybridization test on Plasmodium falciparum infection in human peripheral blood is described. The DNA immobilization both on nylon and nitrocellulose membranes is carry out by UV irradiation. Nonradioactive biotinilated probe is prepared according to original method based on chemical modification of doublest-randed DNA. The correlation coefficient between microscopic examination data and hybridization data is 0.96 for infection density more then 25 parasites by 1000 leukocytes.

References

[1] Application of DNA probes for the diagnosis of malaria: conclusions and recommendations of WHO. Bull. World. Health Organization. 1986. 64(5): 11.
[2] Barker RH Jr, Suebsaeng L, Rooney W, Alecrim GC, Dourado HV, Wirth DF. Specific DNA probe for the diagnosis of Plasmodium falciparum malaria. Science. 1986;231(4744):1434-6.
[3] Franzén L, Westin G, Shabo R, Aslund L, Perlmann H, Persson T, Wigzell H, Pettersson U. Analysis of clinical specimens by hybridisation with probe containing repetitive DNA from Plasmodium falciparum. A novel approach to malaria diagnosis. Lancet. 1984;1(8376):525-8.
[4] Pollack Y, Metzger S, Shemer R, Landau D, Spira DT, Golenser J. Detection of Plasmodium falciparum in blood using DNA hybridization. Am J Trop Med Hyg. 1985;34(4):663-7.
[5] Delves CJ, Goman M, Ridley RG, Matile H, Lensen TH, Ponnudurai T, Scaife JG. Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA. Mol Biochem Parasitol. 1989;32(2-3):105-12.
[6] Aslund L, Franzén L, Westin G, Persson T, Wigzell H, Pettersson U. Highly reiterated non-coding sequence in the genome of Plasmodium falciparum is composed of 21 base-pair tandem repeats. J Mol Biol. 1985;185(3):509-16.
[7] Adarichev VA, Dymshits GM, Kalachikov SM, Pozdniakov PI, Salganik RI. DNA carrying aliphatic amino groups and the use of its fluorescent derivative as a probe in molecular hybridization. Bioorg Khim. 1987;13(8):1066-9.
[8] Kalachikov SM, Adarichev BA, Dymshits GM. Immobilization of DNA on microporous membranes using UV-irradiation. Bioorg Khim. 1992;18(1):52-62.
[9] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[10] Gillespie D, Bresser J. mRNA immobilization in NaI: Quick-blots. BioTechniques. 1983; 1(1):185-92.
[11] Jeanpierre M. A rapid method for the purification of DNA from blood. Nucleic Acids Res. 1987;15(22):9611.
[12] McIntyre P, Stark GR. A quantitative method for analyzing specific DNA sequences directly from whole cells. Anal Biochem. 1988;174(1):209-14.