Biopolymers and cell. 2010; 26 (1): 56 - 61
Optimization of bioselective membrane of amperometric enzyme sensor on basis of glucose oxidase using NH2-modified multi-wall carbone nanotubes
O. A. Biloivan, N. S. Rogaleva, Ya. I. Korpan
Institute of molecular biology and genetics NAS of Ukraine, 150, Zabolotnogo Str, Kyiv Ukraine, 03680
Aim. To investigate a possibility of application of multi-wall carbone nanotubes modified with NH2-groups (MWCNT-NH2) for creation of sensitive elements of the amperometric biosensor based on immobilized oxidoreductases, in particular, glucose oxidase (GOD). To study electrochemical properties of the membranes obtained. Methods. Experiments were carried out with amperometric methods using the ìStat 200 device («DropSens», Spain). The enzymes were immobilised in glutaraldehyde vapour. Results. The method of formation of bioselective matrix based on immobilised GOD with MNP-NH2 on the surface of gold amperometric electrodes was optimised. Optimal working conditions of the biosensor developed were determined. Conclusion. MWCNT integration into a bioselective matrix improves the biosensor analytical characteristics which means: higher signal value, wider linear range of glucose analysis, and possibility of substrate determination in wide range of working potential.
Keywords: NH2-modified multi-wall carbone nanotubes, amperometric biosensor, oxidoreductase, glucose oxidase.