Biopolymers and Cell. 2011; 27(3): 214-220
STUDY ON ASSOCIATION OF THE POLYMORPHIC VARIANTS OF ACE (I/D), AT2R1 (A1166C), TNF (G308A), MTHFR (C677T) GENES AND THEIR COMBINATIONS WITH THE RISK OF DEVELOPMENT OF PERINATAL PATHOLOGY AND GESTATION REDUCTION
P. F. Tatarskyy1, A. M. Kucherenko1, 2, K. G. Khazhilenko3, V. M. Zinchenko3, I. E. Ilyin4, L. A. Livshits1
1Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680
2Taras Shevchenko National University of Kyiv, Institute of Biology
60, Volodymyrska Str., Kyiv, Ukraine, 01033
3 «ISIDA» clinic
65, Ivana Lepse Boulevard, Kyiv, Ukraine, 03126
4 Reproductive Genetics Institute
3d, Zoolohichna St, Kyiv, Ukraine, 03057
Aim. To study association of polymorphisms of the genes of the first CYP1A1 (T6235C) and second GSTM1 («0»), GSTT1 («0»), GSTP1 (A313G) phases of detoxication system as well as polymorphic variants of the genes of proteins of coagulation system F2 (G20210A), F5 (G1691A), and folate metabolism system MTHFR (C677T) with pathogenesis of pregnancy loss. Methods. Polymorphic variants were analyzed using PCR followed by RFLP analysis in 86 healthy individuals and in 109 patients with different types of pregnancy loss. Results. Unfavorable polymorphic variants GSTP1 (313G) and CYP1A1 (6235C) were observed more frequently in the patients with pregnancy loss comparing to the control group. Conclusions. We assume that the 313G polymorphic variant of GSTP1 gene may be considered as a factor of hereditary susceptibility to pregnancy loss.
Keywords: pregnancy loss, detoxication system, blood coagulation system, DNA polymorphism