Biopolymers and Cell. 2011; 27(4): 300-305
MOLECULAR COMPLEXES OF TRITERPENE GLYCOSIDES WITH L-HISTIDINE AND THEIR BIOLOGICAL ACTIVITY
Yakovishin L. A., 1Lekar A. V., 2Vetrova E. V., 1Borisenko N. I., 3Grishkovets V. I.
Sevastopol National Technical University
33, Universitetskaya Str., Sevastopol, Crimea, Ukraine, 99053
1Ecological and Analytical Center, Southern Federal University
7, Zorge Str., Rostov-on-Don, Russian Federation, 344090
2Institute of Physical and Organic Chemistry, Southern Federal University
194/2, Stachka Ave., Rostov-on-Don, Russian Federation, 344090
3V.I. Vernadsky Taurida National University
4, Vernadsky Ave., Simferopol, Crimea, Ukraine, 95007,
Aim. To investigate the complexation of L-histidine (His) with hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-O-α-L-arabino pyranoside (α-hederin) and its 28-O-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl ester (hederasaponin C); to study the effect of glycosides, His, and their mixtures at Avena sativa L. seed germination. Methods. Electrospray ionization mass spectrometry. Results. The mass spectra of glycosides mixed with His are received and interpreted. The comparative analysis of biological activity of individual substances and their complexes is performed. Conclusions. For the first time, the composition of aforesaid complexes is established by mass spectrometry. Complexes have appeared less toxic, than individual glycosides.
Keywords: α-hederin, hederasaponin C, L-histidine, complexation, mass spectrometry, Avena sativa L.