Biopolym. Cell. 1997; 13(5):391-396.
Genome and Its Regulation
2',5'-oligo(A)synthetase activity during early metabolism reorientation in regenerating rat liver
1Obolenskaya M., 2Kelve M., 1Sazonova L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Chemical Physics and Biophysics
    23, Akadeemia tee, Tallinn, Estonia, 12618

Abstract

This paper addresses the verification of our hypothesis about the possible participation of 2' ,5'-oligo(A)synthetase – RNAse L system in the metabolic reorientation of regenerating rat liver during the transit from the quiescence to the proliferation induced by partial hepatectomy (PHE). We analyzed the activity of 2',5'-oligo(A)synthetase, and the dimers/trimers ratios of newly synthesized oligoadenylates. The changes occur in the interval 0.5–3 h after PHE, corresponding to the early period of metabolic reorientation. They reveal the down-regulation of enzymatic activity and the up-regulation of dimers/trimers ratio in nudeoplasm and up-regulation of enzymatic activity in cytoplasm. The temporal correlation of these changes with those previously obtained i. e. with the retain of newly synthesized RNA in the nucleus and with the reorganization of ribosomes on endoplasmatic reticulum supports our hypothesis though does not yet prove it.

References

[1] Higgins GV, Andersen RM. Experimental pathology of the liver. Arch Pathol. 1931; 12(2): 186-202.
[2] Obolenskaia MIu. Age and DNA synthesis in regenerating rat liver. Tsitologiia. 1976;18(7):857-61.
[3] Widmann JJ, Fahimi HD. Proliferation of mononuclear phagocytes (Kupffer cells) and endothelial cells in regenerating rat liver. A light and electron microscopic cytochemical study. Am J Pathol. 1975;80(3):349-66.
[4] Obolenskaya MYu, Prima VI, Gerasimova TB, Platonov OM. Partial restriction of genome expression – a component of its work reprogramming in regenerating liver of mammals. Biopolym Cell. 1989; 5(2):79-88.
[5] Obolenskaya MYu, Prima VI, Kulikovskaya IA, Platonov OM. Nuclear RNA at the early stage of liver regeneration. Biopolym Cell. 1987; 3(1):27-35.
[6] Obolenskaia MIu, Gerasimova TB, Bilich KM, Platonov OM. Intracellular distribution of newly formed hepatocyte RNA in the 1st hours following partial hepatectomy. Tsitol Genet. 1987;21(5):376-82.
[7] Kerr IM, Brown RE. pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells. Proc Natl Acad Sci U S A. 1978;75(1):256-60.
[8] Williams BR, Kerr IM, Gilbert CS, White CN, Ball LA. Synthesis and breakdown of pppA2'p5'A2'p5'A and transient inhibiton of protein synthesis in extracts from interferon-treated and control cells. Eur J Biochem. 1978;92(2):455-62.
[9] Floyd-Smith G, Slattery E, Lengyel P. Interferon action: RNA cleavage pattern of a (2'-5')oligoadenylate--dependent endonuclease. Science. 1981;212(4498):1030-2.
[10] Wreschner DH, McCauley JW, Skehel JJ, Kerr IM. Interferon action--sequence specificity of the ppp(A2'p)nA-dependent ribonuclease. Nature. 1981;289(5796):414-7.
[11] St Laurent G, Yoshie O, Floyd-Smith G, Samanta H, Sehgal PB, Lengyel P. Interferon action: two (2'-5')(A)n synthetases specified by distinct mRNAs in Ehrlich ascites tumor cells treated with interferon. Cell. 1983;33(1):95-102.
[12] Hassel BA, Zhou A, Sotomayor C, Maran A, Silverman RH. A dominant negative mutant of 2-5A-dependent RNase suppresses antiproliferative and antiviral effects of interferon. EMBO J. 1993;12(8):3297-304.
[13] Breeden L, Nasmyth K. Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila. Nature. 1987 Oct 15-21;329(6140):651-4.
[14] Pestka S, Langer JA, Zoon KC, Samuel CE. Interferons and their actions. Annu Rev Biochem. 1987;56:727-77.
[15] Kimchi A, Yarden A, Reznitzky D. Suppression of c-myc by growth inhibitors: the role of endogeneous interferon, and possible cooperation with tumor necrosis factor. Interferons as cell growth inhibitors and antitumor factors. New York: Alan R. Liss, Inc., 1986: 391-402.
[16] Smekens-Etienne M, Goldstein J, Ooms HA, Dumont JE. Variation of (2'-5')oligo(adenylate) synthetase activity during rat-liver regeneration. Eur J Biochem. 1983;130(2):269-73.
[17] Stark GR, Dower WJ, Schimke RT, Brown RE, Kerr IM. 2-5A synthetase: assay, distribution and variation with growth or hormone status. Nature. 1979;278(5703):471-3.
[18] Pivazian AD, Vartanian AA, Zhelkovski? AM, Karpe?ski? MIa. Metabolism of oligoadenylates in cell nuclei and regulation of protein ADP-ribosylation. Mol Biol (Mosk). 1986;20(5):1364-70.
[19] Chebath J, Benech P, Revel M, Vigneron M. Constitutive expression of (2'-5') oligo A synthetase confers resistance to picornavirus infection. Nature. 1987 Dec 10-16;330(6148):587-8.
[20] Busch H. Isolation and purification of nuclei. Methods Enzymol. 1967;421–48.
[21] Hovanessian AG, Svab J, Mari? I, Robert N, Chamaret S, Laurent AG. Characterization of 69- and 100-kDa forms of 2-5A-synthetase from interferon-treated human cells. J Biol Chem. 1988;263(10):4945-9.
[22] Etienne-Smekens M, Vandenbussche P, Content J, Dumont JE. (2'-5')Oligoadenylate in rat liver: modulation after partial hepatectomy. Proc Natl Acad Sci U S A. 1983;80(15):4609-13.
[23] Toots UE, Kel've MB, Saarma MIu. Degradation of messenger RNA, ribosomal RNA and 2-5A-dependent inhibition of protein biosynthesis. Mol Biol (Mosk). 1988;22(6):1473-81.
[24] Schwartz EL, Nilson LA. Activation of 2',5'-oligoadenylate synthetase activity on induction of HL-60 leukemia cell differentiation. Mol Cell Biol. 1989;9(9):3897-903.
[25] Saarma M, Toots U, Raukas E, Zhelkovsky A, Pivazian A, Neuman T. Nerve growth factor induces changes in (2'-5')oligo(A) synthetase and 2'-phosphodiesterase activities during differentiation of PC12 pheochromocytoma cells. Exp Cell Res. 1986;166(1):229-36.
[26] Krause D, Silverman RH, Jacobsen H, Leisy SA, Dieffenbach CW, Friedman RM. Regulation of ppp(A2'p)nA-dependent RNase levels during interferon treatment and cell differentiation. Eur J Biochem. 1985;146(3):611-8.
[27] Jacobsen H, Krause D, Friedman RM, Silverman RH. Induction of ppp(A2'p)nA-dependent RNase in murine JLS-V9R cells during growth inhibition. Proc Natl Acad Sci U S A. 1983;80(16):4954-8.
[28] Cayley PJ, White RF, Antoniw JF, Walesby NJ, Kerr IM. Distribution of the ppp(A2'p)nA-binding protein and interferon-related enzymes in animals, plants, and lower organisms. Biochem Biophys Res Commun. 1982;108(3):1243-50.