Biopolym. Cell. 1997; 13(5):391-396.
Genome and Its Regulation
2',5'-oligo(A)synthetase activity during early metabolism reorientation in regenerating rat liver
1Obolenskaya M., 2Kelve M., 1Sazonova L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Chemical Physics and Biophysics
    23, Akadeemia tee, Tallinn, Estonia, 12618


This paper addresses the verification of our hypothesis about the possible participation of 2' ,5'-oligo(A)synthetase – RNAse L system in the metabolic reorientation of regenerating rat liver during the transit from the quiescence to the proliferation induced by partial hepatectomy (PHE). We analyzed the activity of 2',5'-oligo(A)synthetase, and the dimers/trimers ratios of newly synthesized oligoadenylates. The changes occur in the interval 0.5–3 h after PHE, corresponding to the early period of metabolic reorientation. They reveal the down-regulation of enzymatic activity and the up-regulation of dimers/trimers ratio in nudeoplasm and up-regulation of enzymatic activity in cytoplasm. The temporal correlation of these changes with those previously obtained i. e. with the retain of newly synthesized RNA in the nucleus and with the reorganization of ribosomes on endoplasmatic reticulum supports our hypothesis though does not yet prove it.


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