Biopolym. Cell. 2018; 34(1):41-48.
Viruses and Cell
Genetic characterization of porcine circovirus type 2 (PCV2) from wild boars detected in different regions of Ukraine
1Dudar L. V., 1Budzanivska I. G., 1Polishchuk V. P.
  1. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601

Abstract

Circovirus type 2 are an extremely common swine virus in terms of [is a swine virus extremely widespread in] industrial farming worldwide, including Ukraine]. A similar situation was de-scribed in Ukraine. It leads to an immunosuppressive condition of animals and high mortality of animals. Due to the [Considering a] variety of PCV-2 strains in the world, and the difference be-tween strains common in wildlife and farms, [it is necessary to characterize] the characterization of field isolates found among wild boars in Ukraine is required. So, in this work [the] isolates from wild boars from different regions of Ukraine were characterized and differentiated by phy-logenent [phylogenetic] analysis. As a result of the study, it was shown that [the] isolates from the Chernihiv, Zaporizhzhya, Cherkasy and Kharkiv regions belong to different subgroups of the PCV-2 and have different origin. In addition [Additionally], a relatively high level of similarity with the isolates from Croatia and Brazil has been established. At the same time, the similarity of isolates isolated from wild boars from the Zaporizhzhya and Chernihiv oblasts has been shown to have been isolated from pigs from industrial farms. [At the same time, it has been shown the simi-larity of isolates from wild boars from the Zaporizhzhya and Chernihiv regions with those from pigs of industrial farms.]
Keywords: porcine circovirus of type 2, wild boar, phylogenetic analysis, genetic diversity.

References

[1] Meng XJ. Porcine circovirus type 2 (PCV2): pathogenesis and interaction with the immune system. Annu Rev Anim Biosci. 2013;1:43-64.
[2] Segalés J, Allan GM, Domingo M. Porcine circovirus diseases. Anim Health Res Rev. 2005;6(2):119-42.
[3] Opriessnig T, Meng XJ, Halbur PG. Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Invest. 2007;19(6):591-615.
[4] Cheung AK. Porcine circovirus: transcription and DNA replication. Virus Res. 2012;164(1-2):46-53.
[5] Timmusk S, Wallgren P, Brunborg IM, Wikström FH, Allan G, Meehan B, McMenamy M, McNeilly F, Fuxler L, Belák K, Põdersoo D, Saar T, Berg M, Fossum C. Phylogenetic analysis of porcine circovirus type 2 (PCV2) pre- and post-epizootic postweaning multisystemic wasting syndrome (PMWS). Virus Genes. 2008;36(3):509-20. PubMed PMID: 18343985.
[6] Guo LJ, Lu YH, Wei YW, Huang LP, Liu CM. Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China. Virol J. 2010;7:273.
[7] Kim HK, Luo Y, Moon HJ, Park SJ, Keum HO, Rho S, Park BK. Phylogenetic and recombination analysis of genomic sequences of PCV2 isolated in Korea. Virus Genes. 2009;39(3):352-8.
[8] Franzo G, Tucciarone CM, Cecchinato M, Drigo M. Porcine circovirus type 2 (PCV2) evolution before and after the vaccination introduction: A large scale epidemiological study. Sci Rep. 2016;6:39458.
[9] Jones DT, Taylor WR, Thornton JM. The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci. 1992;8(3):275-82.
[10] Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013;30(12):2725-9.
[11] KKimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16(2):111-20.
[12] Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-25.
[13] Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39(4):783-791.
[14] Grau-Roma L, Crisci E, Sibila M, López-Soria S, Nofrarias M, Cortey M, Fraile L, Olvera A, Segalés J. A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence. Vet Microbiol. 2008;128(1-2):23-35.
[15] Olvera A, Cortey M, Segalés J. Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality. Virology. 2007;357(2):175-85.
[16] Cheung AK, Lager KM, Kohutyuk OI, Vincent AL, Henry SC, Baker RB, Rowland RR, Dunham AG. Detection of two porcine circovirus type 2 genotypic groups in United States swine herds. Arch Virol. 2007;152(5):1035-44.
[17] Li W, Wang X, Ma T, Feng Z, Li Y, Jiang P. Genetic analysis of porcine circovirus type 2 (PCV2) strains isolated between 2001 and 2009: genotype PCV2b predominate in postweaning multisystemic wasting syndrome occurrences in eastern China. Virus Genes. 2010;40(2):244-51.
[18] Trible BR, Rowland RR. Genetic variation of porcine circovirus type 2 (PCV2) and its relevance to vaccination, pathogenesis and diagnosis. Virus Res. 2012;164(1-2):68-77.