Biopolym. Cell. 2002; 18(6):518-521.
Genome and Its Regulation
Gene Cyp2e1 expression in mouse liver cells exposed to low-dose radiation
1Maksymchuk O. V., 1Danko I. M., 1Rosohac'ka I. V., 1Chaschyn M. O.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


This study presents results on gene Cyp2e1 expression peculiarities in mouse liver cells under continued exposure to low-dose radiation. The gene Cyp2e1 expression and cytochrome P450 relative content were shown to decrease under this exposure. Furthermore, the gene Cyp2e1 expression level was found to depend on the gender and age of animals. The differential expression levels of hepatic gene Cyp2e1 in the mature mice, suggest that regulation occurs at a transcriptional or posttranslational level


[1] Parke DV, Ioannides C, Lewis DF. The 1990 Pharmaceutical Manufacturers Association of Canada keynote lecture. The role of the cytochromes P450 in the detoxication and activation of drugs and other chemicals. Can J Physiol Pharmacol. 1991;69(5):537-49.
[2] Nebert DW, Nelson DR, Coon MJ, Estabrook RW, Feyereisen R, Fujii-kuriyama Y, et al. The P450 superfamily: update on new sequences, gene mapping, and recommended nomenclature. DNA and Cell Biology. 1991;10(1):1–14.
[3] Emoto C, Yamazaki H, Yamasaki S, Shimada N, Nakajima M, Yokoi T. Characterization of cytochrome P450 enzymes involved in drug oxidations in mouse intestinal microsomes. Xenobiotica. 2000;30(10):943–53.
[4] Freeman JE, Stirling D, Russell AL, Wolf CR. cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2e1. Biochem J. 1992;281 ( Pt 3):689-95.
[5] Gonzalez FJ. Molecular genetics of the P450 superfamily. Pharmacol Ther. 1990; 45: 1-38.
[6] Paramonova GI. [Age and the effects of barbiturates on rat liver microsome oxidative enzymes]. Farmakol Toksikol. 1981;44(1):98-101.
[7] Ariyoshi T, Tsuboi K, Hamasaki K. Effects of age and sex on microsomal heme oxygenase and cytochrome P-450 content in liver of rats. J Pharmacobiodyn. 1981;4(9):664-9.
[8] Porter TD, Coon MJ. Cytochrome P-450. Multiplicity of isoforms, substrates, and catalytic and regulatory mechanisms. J Biol Chem. 1991;266(21):13469-72.
[9] Prima VI, Glushakova LG. Radiation-induced peculiarities of cytochrome P-4502E1 and oncogenes mRNA accumulation in different rat tissues. Biopolym Cell. 1998; 14(6):540-3.
[10] Glushakova LG, Maksimchuk OV, Danko IM, Siniuk OF, Chashchin NA. [Effect of factors from the Chernobyl exclusion zone on the level of cytochrome P-450 in mouse liver microsomes]. Ukr Biokhim Zh. 2000;72(6):63-6.
[11] Omura T, Sato R. The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem. 1964;239:2370-8.
[12] Peterson GL. Determination of total protein. Methods Enzymol. 1983;91:95-119.
[13] Peppel K, Baglioni C. A simple and fast method to extract RNA from tissue culture cells. Biotechniques. 1990;9(6):711-3.
[14] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning. A laboratory manual. New York: Cold Spring Harbour Lab. press, 1982. 452 p.
[15] Barabo? VA, Oli?nik SA, Khmielievsky? IuV. [Status of the antioxidant system at low doses and low intensities of ionizing radiation]. Ukr Biokhim Zh. 1994;66(4):3-18.