Biopolym. Cell. 2002; 18(4):287-296.
http://dx.doi.org/10.7124/bc.00060D
Структура та функції біополімерів
Аналіз взаємодії антибіотика мітоксантрону з ДНК у присутності кофеїну у водному розчині за даними 1Н-ЯМР спектроскопії
1Веселков О. Н., 1Висоцький С. О., 1Євстигнєєв М. П., 2Веселков Д. О., 1Димант Л. Н., 1Болотін П. О., 2Девіс Д. Б.
  1. Севастопольський національний технічний университет
    вул. Університетська, 33, Севастополь, Україна, 99053
  2. Беркбек колледж Лондонского университета
    Малет-стрит, Лондон WC1E 7НХ, Великобритания

Abstract

Методом одно- і двовимірної 1Н-ЯМР спектроскопії (500 МГц) вивчено зв'язування протипухлинного антибіотика мітоксантрону, або новантрону (NOV), з ДНК у присутності кофеїну (CAF) у водному розчині. Комплексоутворення моле­кул проналізовано з урахуванням термодинамічних парамет­рів реакцій само- і гетероасоціації NOV і CAF та взаємодії лігандів з дезокситетрануклеотидом 5'-d(TpCpGpA) за одна­кових експериментальних умов. Зроблено висновок про сиквенс-специфічність зв'язування NOV з піримідин-пуриновими послідовностями досліджуваного олігонуклеотиду. Показано, що зниження ефективності NOV, інтеркалюючого в ДНК при додаванні до розчину CAF, у значній мірі пов'язано з блокуван­ням молекулами
Повний текст: (PDF, російською)

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