Biopolym. Cell. 1989; 5(3):39-43.
Структура та функції біополімерів
Вплив іонної сили розчину на енергію надспіралізації ДНК
1Шурдов М. А., 1Груздев О. Д.
  1. Інститут цитології і генетики, Сибірського відділення АН СРСР
    Новосибірськ, СРСР

Abstract

Визначено залежність вільної енергії надспіралізації g > Kσ2 від концентрації NaCl у розчині за вимірюваннями ізотерм зв’язування бромистого етидію з ДНК плазміди pBR322. Показано, що зі зменшенням концентрації солі від 0,3 до 0,01 M значення константи К зростає більш ніж удвічі, а кількість надвитків помітно падає. Енергія надспіралізації ДНК зменшується приблизно на 20 %.

References

[1] Gragerob AI, Mirkin SM. Influence of DNA superhelicity on the major genetic processes in prokaryotes. Mol Biol (Mosk). 1980;14(1):8-34.
[2] Kmiec EB, Worcel A. The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts. Cell. 1985;41(3):945-53.
[3] Fisher LM. DNA supercoiling and gene expression. Nature. 1984 Feb 23-29;307(5953):686-7.
[4] Lazurkin YuS. DNA: supercoiling and alternative structures. Biopolym. Cell. 1986; 2(6):283-92.
[5] Wang JC, Peck LJ, Becherer K. DNA supercoiling and its effects on DNA structure and function. Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:85-91.
[6] Singleton CK. Effects of salts, temperature, and stem length on supercoil-induced formation of cruciforms. J Biol Chem. 1983;258(12):7661-8.
[7] Singleton CK, Klysik J, Stirdivant SM, Wells RD. Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions. Nature. 1982;299(5881):312-6.
[8] Hinton DM, Bode VC. Ethidium binding affinity of circular lambda deoxyribonucleic acid determined fluorometrically. J Biol Chem. 1975;250(3):1061-70.
[9] Upholt WB, Gray HB Jr, Vinograd J. Sedimentation velocity behavior of closed circular SV40 DNA as a function of superhelix density, ionic strength, counterion and temperature. J Mol Biol. 1971;62(1):21-38.
[10] Pulleyblank DE, Shure M, Tang D, Vinograd J, Vosberg HP. Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers. Proc Natl Acad Sci U S A. 1975;72(11):4280-4.
[11] Depew DE, Wang JC. Conformational fluctuations of DNA helix. Proc Natl Acad Sci U S A. 1975;72(11):4275-9.
[12] Bauer W, Vinograd J. Interaction of closed circular DNA with intercalative dyes. II. The free energy of superhelix formation in SV40 DNA. J Mol Biol. 1970;47(3):419-35.
[13] Lee CH, Mizusawa H, Kakefuda T. Unwinding of double-stranded DNA helix by dehydration. Proc Natl Acad Sci U S A. 1981;78(5):2838-42.
[14] LePecq JB, Paoletti C. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol. 1967;27(1):87-106.
[15] Borisova OF, Grechko VV, Kuznetsova NV, Sakharova NK, Timokhina GI. Comparison of the conformation of RNA from phage MS2 and 16S rRNA. Interaction with dyes specific for the secondary structure of native RNA and RNA subjected to hydrolysis by nuclease S1. Mol Biol (Mosk). 1987;21(2):515-28.
[16] Hsieh TS, Wang JC. Thermodynamic properties of superhelical DNAs. Biochemistry. 1975;14(3):527-35.
[17] Sen S, Majumdar R. Thermodynamics of B-Z transition in supercoiled DNA. Nucleic Acids Res. 1987;15(14):5863-71.
[18] Millar DP, Robbins RJ, Zewail AH. Torsion and bending of nucleic acids studied by subnanosecond time-resolved fluorescence depolarization of intercalated dyes. J Chem Phys. 1982;76(4):2080–94.
[19] Hagerman PJ. Investigation of the flexibility of DNA using transient electric birefringence. Biopolymers. 1981;20(7):1503-35.
[20] Yarmola EG, Zarudnaya MI, Lazurkin YuS. Dependence of osmotic pressure of DNA solution and effective diameter on the ionic strength. Biofizika. 1986; 31(2):338-9.
[21] Stigter D. Interactions of highly charged colloidal cylinders with applications to double-stranded. Biopolymers. 1977;16(7):1435-48.
[22] Brian AA, Frisch HL, Lerman LS. Thermodynamics and equilibrium sedimentation analysis of the close approach of DNA molecules and a molecular ordering transition. Biopolymers. 1981;20(6):1305-28.
[23] Shore D, Baldwin RL. Energetics of DNA twisting. II. Topoisomer analysis. J Mol Biol. 1983;170(4):983-1007.
[24] Horowitz DS, Wang JC. Torsional rigidity of DNA and length dependence of the free energy of DNA supercoiling. J Mol Biol. 1984;173(1):75-91.
[25] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual New York: Cold Spring Harbor Lab. publ., 1982 545 p.
[26] Wang JC. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients. J Mol Biol. 1974;89(4):783-801.