Biopolym. Cell. 1991; 7(4):6-20.
http://dx.doi.org/10.7124/bc.0002DA
Клітинна біологія
Індивідуальне культивування преселектованих рослинних протопластів і продуктів їхнього злиття у мікрокраплях
1Кириченко І. В., 1Глеба Ю. Ю.
  1. Інститут клітинної біології та генетичної інженерії АН УСРС
    Київ, СРСР

Abstract

Показано успішний розвиток одиничних протопластів і клітин, а також продуктів злиття одиничних преселектованих протопластів різних видів рослин за допомогою розробленого авторами методу індивідуального культивування у мікрокраплях. Ефективність висіву складала від 2 (конюшина) до 75 % (тютюн), ефективність електрозлиття одиничних протопластів сягала 95 %. Описано застосовану технологію електрозлиття, запропоновано нову процедуру додання культурального середовища клітинам, що знаходяться в мікрокраплях, яка доволяє значно підвищити ефективність методу.
Повний текст: (PDF, російською)

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