Biopolym. Cell. 1985; 1(2):79-86.
Cell Biology
Inhibition of replicative DNA synthesis in nuclei of sea urchin (Strongylocentrotus intermwium) embryo by 2', 3'-dideoxy-3'-aminonucleoside 5'-triphosphates
1Kukhanova M. K., 1Kraevsky A. A., 2Terentieva N. A., 2Rasskazov V. A.
  1. Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR
  2. The Pacific Ocean Institute of Bioorganic Chemistry, Academy of Sciences of the USSR,
    Vladivostok, USSR


2', 3'-dideoxy-3'-aminonucleoside-5'-triphosphates are shown to be inhibitors of repllcative DNA synthesis in isolated nuclei of sea urchin embryo. These compounds inhibit the fragment Okazaki synthesis. The effect of 2', 3'-dideoxy-3'-aminothymidine 5'-triphosphate and arabinothymidine 5'-triphosphate is reversible when adding the corresponding substrate of DNA synthesis, 2'-deoxy-thymidine-5'-triphosphate.


[1] Chidgeavadze ZG, Beabealashvilli RS, Atrazhev AM, Kukhanova MK, Azhayev AV, Krayevsky AA. 2',3'-Dideoxy-3' aminonucleoside 5'-triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases. Nucleic Acids Res. 1984;12(3):1671-86.
[2] Kukhanova MK, Kochetkova SV, Kraevskii AA, Terent'ev LL, Rasskazov VA. Inhibition of DNA biosynthesis in sea urchin embryos by 2',3'-dideoxy-3'-aminonucleosides. Biokhimiia. 1983;48(10):1747-51.
[3] Zaitseva V. E., Dyatkina N. B., Krayevsky A. A., Skaptsova N. V., Turina O. V., Gnuchev N. V., Gottikh B. P., Azhayev A. V. Aminonucleosides and their derivatives. XI. Synthesis of 3'-amino-2',3'-dideoxynucleoside 5'-triphosphates. Russian Journal of Bioorganic Chemistry. 1984;10(5):670-680.
[4] Schinazi RF, Chen MS, Prusoff WH. Antiviral and antineoplastic activities of pyrimidine arabinosyl nucleosides and their 5'-amino derivatives. J Med Chem. 1979;22(10):1273-7.
[5] Oguro M, Shioda M, Nagano H, Mano Y, Hanaoka F, Yamada M. The mode of action of aphidicolin on DNA synthesis in isolated nuclei. Biochem Biophys Res Commun. 1980;92(1):13-9.
[6] Cozzarelli NR. The mechanism of action of inhibitors of DNA synthesis. Annu Rev Biochem. 1977;46:641-68.
[7] Butt TR, Wood WM, McKay EL, Adams RL. Involvement of deoxyribonucleic acid polymerase beta in nuclear deoxyribonucleic acid synthesis. Biochem J. 1978;173(1):309-14.
[8] Zoncheddu A, Aceomando R, Badaraceo G. Comparison of the effects of differential inhibitors of eucaryotic DNA polymerases on DNA synthesis in isolated rat liver nuclei. Int J Biochem. 1983; 15(3):337-342.
[9] Wist E, Krokan H, Prydz H. Effect of 1-beta-D-arabinofuranosylcytosine triphosphate on DNA synthesis in isolated HeLa cell nuclei. Biochemistry. 1976;15(16):3647-52.
[10] Waqar MA, Evans MJ, Huberman JA. Effect of 2',3'-dideoxythymidine-5'-triphosphate on HeLa cell in vitro DNA synthesis: evidence that DNA polymerase alpha is the only polymerase required for cellular DNA replication. Nucleic Acids Res. 1978;5(6):1933-46.
[11] Berger NA, Johnson ES. DNA synthesis in permeabilized mouse L cells. Biochim Biophys Acta. 1976;425(1):1-17.