Biopolym. Cell. 1985; 1(2):86-91.
Cell Biology
Expression of T-region genes in somatic cell hybrids obtained by fusion of normal and tumour cells
1Kanevsky I. F., 1Cherep N. N., 1Skarzhynskaya M. V., 1Gleba Yu. Yu.
  1. N. G. Kholodny Institute of Botany, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Protoplast fusion of Nicotiana tabacum (56S3) tumour cells and Atropa belladonna L. mesophyll leaf cells was carried out. 57 hybrid cell lines in hormone-free medium are selected. Analysis of hybrid lines involved the determination of the multiple molecular forms of amylase and the LpDH activity, suppression of shoot and root organogenesis, resistance to 5-bromodeoxyuridine, 2-aminoethylcystein, 5-methyltryptophan as well as cytogenetic study. The available data permit demonstrating that under somatic cell hybridization most of tumour characters, controlled by T-DNA, are inherited as dominant. In some cases the expression of these characters depends on exogenous phytohormone presence or absence in nutrient media.


[1] White PR, Braun AC. A cancerous neoplasm of plants. Autonomous bacteria-free crown-gall tissue. Cancer Res. 1942; 2:597-617.
[2] Lioret C. Les acides amines libres des tissus de crown-gall cultives in vitro. Mise en evidence d'un acide amine particulier a ces tissus. C. R. Acad. Sci. Paris, 1957; 244:2171-2174.
[3] Menage A, Morel G. Sur la presence d'octopine dans les tissus de crown-gall. C. R. Acad. Sci. Paris. 1964; 259:4795-4796.
[4] Kemp JD. A new amino acid derivative present in crown gall tumor tissue. Biochem Biophys Res Commun. 1977;74(3):862-8.
[5] Wullems GJ, Molendijk L, Schilperoort RA. The expression of tumour markers in intraspecific somatic hybrids of normal and crown gall cells from Nicotiana tabacum. Theor Appl Genet. 1980;56(5):203-8.
[6] Kanevskiy IF, Gleba YUYU, Cherep NN. Obtaining and analysis of cell hybrids between Nicotiana plumbaginifolia and Nicotiana tabacum transformed with Agrobacterium tumafaciens. Genetika. 1983;19(12):2060-6.
[7] Linsmaier EM, Skoog F. Organic growth factor requirements of tobacco tissue cultures. Physiol Plantarum. 1965;18(1):100-127.
[8] Gleba YY, Hoffmann F. Hybrid cell lines Arabidopsis thaliana + Brassica campestris: No evidence for specific chromosome elimination. Mol Gen Genet., 1978; 165(3):257-64.
[9] Otten LA, Schilperoort RA. A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities. Biochim Biophys Acta. 1978;527(2):497-500.
[10] Davis BJ. Disc electrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci. 1964;121:404-27.
[11] Menczel L, Nagy F, Kiss ZR, Maliga P. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids. Theor Appl Genet. 1981;59(3):191-5.
[12] Gleba YY, Momot VP, Cherep NN, Skarzynskaya MV. Intertribal hybrid cell lines of Atropa belladonna (X) Nicotiana chinensis obtained by cloning individual protoplast fusion products. Theor Appl Genet. 1982;62(1):75-9.
[13] Dahl GA, Tempe J. Studies on the use of toxic precursor analogs of opines to select transformed plant cells. Theor Appl Genet. 1983;66(3-4):233-9.
[14] Meitis F. 5-Bromodeoxyuridine: A specific inhibitor of cytokinin-habituation in tobacco cell culture. Planta. 1976;129(3):239-44.
[15] Vyskot B, Karpfel Z, Bezdek M. Hormonal control of 5-bromodeoxyuridine (BUdR) tolerance in crown-gall tumors and habituated tissues. Planta. 1977;137(3):247-52.