Biopolym. Cell. 1985; 1(2):92-98.
Viruses and Cell
Absence of the avian sarcoma virus genes in the transformed mammalian cells
1Rynditch A. V., 2Gerik I., 2Lgotak V., 1Yatsula B. A., 2Svoboda Ya.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Institute of Molecular Genetics, Czechoslovak Academy of Sciences
    Prague, Czechoslovak


DNA from six lines of mouse and syrian hamster tumour cells induced by Rous sarcoma virus (RSV), its XC RSV variant and recovered avian sarcoma virus 2257 (rASV) are characterized by the absence of viral gene sequences though these cells retain their oncogenic phenotype while passing. The deletion of transforming gene src suggests that in some cases its presence is not needed for maintenance of transformation. In two tumour cell lines RSV long terminal repeat sequences (RSV-LTR) are revealed. DNA from the hamster tumour cell lines induced by rASV 2257 contains the altered cellular oncogene c-Kl-ras. Both RSV LTR-sequences and altered c-Ki-ras gene may participate in the formation and maintenance of H-2257 hamster tumours.


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