Biopolym. Cell. 1985; 1(4):213-218.
Biopolymers and Genome Regulation
Steroid-receptor complexes and mechanism for regulation of the eucaryotic genome transcription
1Romanov G. A.
  1. K. A. Timiryazev Institute of Plant Physiology, Academy of Sciences of the USSR
    Moscow, USSR


The in vitro and in vivo interaction of steroid-receptor complexes with nucleic acids and chromatin is studied. The highly purified glucocorticoid-receptor complex is shown to possess a pronounced selectivity of its association with nucleic acids depending on their base composition and sequence. It is stated that the rat liver glucocorticoid receptors bind to definite sites of homologous DNA with an increased affinity; these sites are involved into the of transcriptionaly active chromatin under the effect of in vivo glucocorticoids on living cells. Studies of DNA methylation level in rat liver and its chromatin sensitivity to DNAase I during hormonal induction, have permitted concluding that hormone-responsive chromatin loci possess a distinct В«potentially active conformation. Thus, hormonal responsiveness of genes depends on DNA sequence as well as on the way of their organization within chromatin. Intracellular RNA may compete with DNA for receptor binding and thereby moderate the intensity of hormonal influence on chromatin.


[1] Romanov GA, Vaniushin BF. Methylation of DNA in eukaryotes. I. Methylated sequences and DNA methylases. Nauchnye Doki Vyss Shkoly Biol Nauki. 1980;(11):5-20.
[2] Razin A, Riggs AD. DNA methylation and gene function. Science. 1980;210(4470):604-10.
[3] Romanov GA, Vaniushin BF. Methylation of eukaryote DNA. II. Biological significance. Nauchnye Doki Vyss Shkoly Biol Nauki. 1981;(1):5-13.
[4] Rosen VB, Smirnov AN receptors and steroid hormones. Moscow: Izd. University Press, 1981. 310.
[5] Romanov GA, Sokolova NA, Rozen VB, Vaniushin BF. Interaction of dexamethasone-receptor complexes with rat liver nuclei and DNAs. Biokhimiia. 1976;41(12):2140-9.
[6] Romanova NA, Romanova GA, Rozen VB, Vaniushin BF. Interaction of rat liver dexamethasone-receptor complexes with DNA. Biokhimiia. 1979;44(3):529-42.
[7] Romanov GA, Romanova NA, Rozen VB, Vaniushin BF. Glucocorticoid-receptor complexes of rat liver. I. Kinetic and equilibrium parameters of their interaction with DNA: ionic strength and temperature dependence. Mol Biol (Mosk). 1981;15(3):601-12.
[8] Romanov GA, Romanova NA, Rozen VB, Vaniushin BF. Glucocorticoid-receptor complexes of rat liver. II. Interaction with natural and synthetic polynucleotides. Mol Biol (Mosk). 1981;15(4):857-74.
[9] Romanov GA, Romanova NA, Rozen VB, Vanyushin BF. Interaction of purified rat liver glucocorticoid-receptor complexes with polynucleotides: strong base composition dependence. Biochem Int. 1983;6(3):339-48.
[10] Romanova NA, Romanov GA, Rozen VB, Vaniushin BF. Reaction between dexamethasone-receptor complexes and DNA as the basis for their translocation into the cell nucleus in vivo. Dokl Akad Nauk SSSR. 1978;243(6):1585-8.
[11] Rennie PS. Binding of androgen receptor to prostatic chromatin requires intact linker DNA. J Biol Chem. 1979;254(10):3947-52.
[12] Payvar F, Wrange O, Carlstedt-Duke J, Okret S, Gustafsson JA, Yamamoto KR. Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo. Proc Natl Acad Sci U S A. 1981;78(11):6628-32.
[13] Govindan MV, Spiess E, Majors J. Purified glucocorticoid receptor-hormone complex from rat liver cytosol binds specifically to cloned mouse mammary tumor virus long terminal repeats in vitro. Proc Natl Acad Sci U S A. 1982;79(17):5157-61.
[14] Pfahl M. Specific binding of the glucocorticoid-receptor complex to the mouse mammary tumor proviral promoter region. Cell. 1982;31(2 Pt 1):475-82.
[15] Kuz'menko AP, Dashkevich VS, Romanov GA, Vaniushin BF, Salganik RI. Transition of the specific binding sites of glucocorticoid receptor complexes from the fraction of potentially active DNA into the fraction of transcriptionally active DNA under cortisol induction in rat liver cells. Dokl Akad Nauk SSSR. 1983;272(3):739-41.
[16] Romanov GA, Kuzmenko AP, Dashkevich VS, Salganik RI, Vanyushin BF. Purified glucocorticoid-receptor complexes from rat liver cytosol preferentially bind in vitro to a homologous DNA fraction whose transcription is activated by cortisol. FEBS Lett. 1984;165(1):35-8.
[17] Lino S, Smythe S, Tymoczko JL, Rossini GP, Chen C, Hiipakka RA. RNA-dependent release of androgen and other steroidreceptor complexes from DNA. J Biol Chem. 1980;255(12):5545-51.
[18] Compton JG, Schrader WT, O'Malley BW. Selective binding of chicken progesterone receptor A subunit to a DNA fragment containing ovalbumin gene sequences. Biochem Biophys Res Commun. 1982;105(1):96-104.
[19] Mulvihill ER, LePennec JP, Chambon P. Chicken oviduct progesterone receptor: location of specific regions of high-affinity binding in cloned DNA fragments of hormone-responsive genes. Cell. 1982;28(3):621-32.
[20] Compton JG, Schrader WT, O'Malley BW. DNA sequence preference of the progesterone receptor. Proc Natl Acad Sci U S A. 1983;80(1):16-20.
[21] Romanov GA, Gorskova VA. Purification and comparative study of the properties of glucocorticoid- and estrogen-receptor complexes in the rat liver. Biokhimiia. 1984;49(1):3-11.
[22] Luksha GL, Romanov GA. Participation of chromatin proteins in the binding of glucocorticoid receptor complexes by the cell nucleus. Nauchnye Doki Vyss Shkoly Biol Nauki. 1984;(4):22-6.
[23] Simpson R. T. Dissection of chromatin structure with nucleases. In: Gene amplification and analysis. Structural analysis of nucleic acids. N. Y., Amsterdam, Oxford: Elsevier, 1981, vol. 2:347-368.
[24] Gasson JC, Ryden T, Bourgeois S. Role of de novo DNA methylation in the glucocorticoid resistance of a T-lymphoid cell line. Nature. 1983;302(5909):621-3.
[25] Romanov GA, Zhavoronkova EN, Savel'ev SV, Vaniushin BF. Nuclease sensitivity and methylation of chromatin DNA of the rat liver in the initial period of glucocorticoid activity. Probl Endokrinol (Mosk). 1984;30(6):38-42.
[26] Dashkevich VS, Kuzmenko AP, Skobeleva NA. etc. Transition globin gene is transcriptionally active fraction of DNA in a fraction of potentially active DNA during differentiation of reticulocytes into erythrocytes in birds. Dokl Akad Nauk SSSR. 1983; 270(5):1236-8.
[27] Romanov GA, Vanyushin BF. Cytosol induces apparent selectivity of glucocorticoid receptor binding to nucleic acids of different secondary structure. Biochim Biophys Acta. 1982;699(1):53-9.
[28] Romanov GA. Possible function of RNA-binding proteins of eukaryotes: blocking of RNA accessibility for DNA-interacting (regulatory) protein. Biokhimiia. 1982;47(3):339-42.