Biopolym. Cell. 1985; 1(4):219-224.
Gene-Engineering Biotechnology
Introduction of mu bacteriophage into Azotobacter chroococcum and intergeneric rp4 :: mu plasmid-mediated transfer of genes
1Raschinkina A. S., 1Troitsky N. A., 1Okulich L. A.
  1. Institute of Genetics and Cytology, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR


Recombinant plasmid of RP4:: Mu cts62 is constructed. The plasmid is transferred by conjugation with the 10-3-10-4 frequency from E. coli to taxonomically nonrelated bacterium of Azotobacter chroococcum. Azotobacter strains are revealed where Mu cts62 phage DNA was replicated and about 105 plaque-forming units per ml-1 was produced. The strain of A. chroococcum 31/8 (RP4 :: Mu cts62) is used as a donor of chromosomal genes in intergeneric mating with E. coli AB1157 recipient. The RP4 :: Mu cts62 plasmid-mediated transfer of thr and his markers is found.


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