Biopolym. Cell. 1985; 1(4):219-224.
Gene-Engineering Biotechnology
Introduction of mu bacteriophage into Azotobacter chroococcum and intergeneric rp4 :: mu plasmid-mediated transfer of genes
1Raschinkina A. S., 1Troitsky N. A., 1Okulich L. A.
  1. Institute of Genetics and Cytology, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR

Abstract

Recombinant plasmid of RP4:: Mu cts62 is constructed. The plasmid is transferred by conjugation with the 10-3-10-4 frequency from E. coli to taxonomically nonrelated bacterium of Azotobacter chroococcum. Azotobacter strains are revealed where Mu cts62 phage DNA was replicated and about 105 plaque-forming units per ml-1 was produced. The strain of A. chroococcum 31/8 (RP4 :: Mu cts62) is used as a donor of chromosomal genes in intergeneric mating with E. coli AB1157 recipient. The RP4 :: Mu cts62 plasmid-mediated transfer of thr and his markers is found.

References

[1] Denarie J, Rosenberg C, Bergeron B et al. Potential of RP4:: Mu plasmids for in vivo genetic engineering of gram-negative bacteria. In: DNA insertion elements, plasmids and episomes. N. Y.: Cold Spring Harbor, 1977, p. 507-520.
[2] Murooka Y, Takizawa N, Harada T. Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu. J Bacteriol. 1981;145(1):358-68.
[3] Beacham IR, Garrett S. Transfer of RP4::Mu to Salmonella typhimurium. J Gen Microbiol. 1981;124(1):225-8.
[4] Kameneva SV, Polivtseva TP, Kopteva AV, Muronets EM. Conjugation in Rhodopseudomonas sphaeroides mediated by R plasmids. Genetika. 1982;18(9):1433-41.
[5] Perombelon M. C. M., Boucher C. Developing of mating system in Erwinia carotovora. In: Proc. 4th Int. Conf. Plant Pathog. Bact. Angers, 1978; 1:47-52.
[6] Van Gijsegem F, Toussaint A. Chromosome transfer and R-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, and Proteus mirabilis. Plasmid. 1982;7(1):30-44.
[7] Schoonejans E, Toussaint A. Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi. J Bacteriol. 1983;154(3):1489-92.
[8] Lejeune P, Mergeay M, Van Gijsegem F, Faelen M, Gerits J, Toussaint A. Chromosome transfer and R-prime plasmid formation mediated by plasmid pULB113 (RP4::mini-Mu) in Alcaligenes eutrophus CH34 and Pseudomonas fluorescens 6.2. J Bacteriol. 1983;155(3):1015-26.
[9] Sadoff HL, Shimel B, Ellis S. Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes. J Bacteriol. 1979;138(3):871-7.
[10] Taylor AL. Bacteriophage-induced mutation in Escherichia coli. Proc Natl Acad Sci U S A. 1963;50:1043-51.
[11] Boucher C, Bergeron B, De Bertalmio MB, D?nari? J. Introduction of bacteriophage Mu into Pseudomonas solanacearum and Rhizobium meliloti using the R factor RP4. J Gen Microbiol. 1977;98(1):253-63.
[12] Raschynkina A. C., Troitsky M. A., Akulich LA transfer plasmid RP4 at kanyugatsii and its gene expression in Azotobacter chroococcum. Vesti Acad Sciences of Belarus, Ser biology Science. 1981; 3:64-68.
[13] van Vliet F, Silva B, van Montagu M, Schell J. Transfer of RP4::mu plasmids to Agrobacterium tumefaciens. Plasmid. 1978;1(4):446-55.
[14] Tucker WT, Pemberton JM. The introduction of RP4 :: Mu cts62 into Rhodopseudomonas sphaeroides. FEMS Microbiol Lett. 1979; 5(3):215-217.
[15] Clarke L, Carbon J. Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations. J Mol Biol. 1978;120(4):517-32.