Biopolym. Cell. 1985; 1(5):271-276.
Short Communications
The use of the Okayama-Berg vector system principle for barley cDNA cloning
1Dyachenko L. F., 1Sivolap Yu. M.
  1. All-Union Breeding-Genetic Institute,
    V. I. Lenin All-Union Academy of Agricultural Sciences
    Odessa, USSR


A new approach is stated for obtaining full size cDNA molecules which is based on the Okayama-Berg cloning technique. The properties of poly/A/+mRNA from barley endosperms and scheme of obtaining cDNA are described. This cDNA was inserted in plasmid and cloned. Transformation allowed selecting about 2x103 clones.


[1] Bartels D, Thompson RD. The characterization of cDNA clones coding for wheat storage proteins. Nucleic Acids Res. 1983;11(10):2961-77.
[2] Ryskov AP, Timchenko NA, Timchenko LT, Salikhov TA, Gaitsokhi VS. Cloning of double-stranded DNA--a transcript of rat transferrin mRNA. Mol Biol (Mosk). 1984;18(1):104-14.
[3] Rabbitts TH. Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA. Nature. 1976;260(5548):221-5.
[4] Wood KO, Lee JC. Integration of synthetic globin genes into an E. coli plasmid. Nucleic Acids Res. 1976;3(8):1961-71.
[5] Sleigh MJ, Both GW, Brownlee GG. The influenza virus haemagglutinin gene: cloning and characterisation of a double-stranded DNA copy. Nucleic Acids Res. 1979;7(4):879-93.
[6] Rose JK. Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus. Cell. 1980;19(2):415-21.
[7] Friedman EY, Rosbash M. The syntheiss of high yields of full-length reverse transcripts of globin mRNA. Nucleic Acids Res. 1977;4(10):3455-71.
[8] Chang AC, Nunberg JH, Kaufman RJ, Erlich HA, Schimke RT, Cohen SN. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. Nature. 1978;275(5681):617-24.
[9] Efstratiadis A, Kafatos FC, Maniatis T. The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell. 1977;10(4):571-85.
[10] Sood AK, Pereira D, Weissman SM. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc Natl Acad Sci U S A. 1981;78(1):616-20.
[11] Rougeon F, Kourilsky P, Mach B. Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid. Nucleic Acids Res. 1975;2(12):2365-78.
[12] Land H, Grez M, Hauser H, Lindenmaier W, Sch?tz G. 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Res. 1981;9(10):2251-66.
[13] Okayama H, Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982;2(2):161-70.
[14] Beachy RN, Barton KA, Madison JT. et al. The mRNAs that code for soybean seed protein . In: Genome organization and expression in plants. N. Y.: Plenum Press, 1980, p. 273-289.
[15] Hall TC, Ma Y, Buchbinder BU, Pyne JW, Sun SM, Bliss FA. Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization. Proc Natl Acad Sci U S A. 1978;75(7):3196-200.
[16] Brandt A, Ingversen J. In vitro synthesis of barley endosperm proteins on wild type and mutant templates. Carlberg Res. Commun., 1976; 41(6):311-320.
[17] Miflin B, Matthews JA, Burgess SR. et al. The synthesis of barley storage proteins . In: Genome organization and expression in plants. N. Y.: Plenum Press, 1980, p. 233-243.