Biopolym. Cell. 1985; 1(6):283-292.
Oligonucleotide sequences selectively bound by Escherichia coli RNA polymerase
1Knorre V. L., 1Savinkova L. K., 1Salganik R. I.
  1. Institute of Cytology and Genetics, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR


It is found that E. coli RNA polymerase selectively binds certain oligonucleotides from the random isoplith mixtures obtained by nuclease digestion of either DNA or RNA. The binding is realized only by catalytically active enzyme under conditions optimal for transcription. Using syntetic oligodeoxyribonucleotides of definite sequences is shown that the efficient binding occurs with oligonucleotides related to the «—10» promoter region comprising the Pribnow box and downstream flankng nucleotides up to the transcription initiation site. Oligonucleotides provided with 5'-terminal aklylating groups are covalently bound to RNA polymerase, σ-subunit being labelled with deoxyribonucleotide derivative and β,β'-subunits — with ribooligonucleotide ones.


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