Biopolym. Cell. 1994; 10(6):72-78.
Interaction between feeder and human bone marrow stromal precursor cells during cloning
1Astakhova V. S., 1Taran E. V., 1Panchenko L. M.
  1. Ukrainian Scientific Research Institute of Traumatology and Orthopedics
    27, Vorovskogo, Kyiv, Ukraine, 01601


It has been studied interaction between feeder cells and human bone marrow colony forming units of fibroblasts (CFUf) during cloning in vitro. It has been determined that CFUf is sensitive to action of feeder only in 1–3 days after explantation. Nonadhesive fraction of bone marrow cells is responsible for feeder effect. Direct cell-cell interactions play the principal role in the influence of feeder on CFUf. In their absence bone marrow CFUf loses the ability to form the colony.


[1] Canalis E. Bone related growth factors. Triangl. 1988; 27(12):11—19.
[2] Friedenstein AJ, Luria EA Cellular basis of hematopoietic microenvironment. M: Meditsina. 1980. 216 p.
[3] Friedenstein AJ, Lalykina KS. Induction of bone tissue and osteogenic progenitor cells. M.: Meditsina, 1973. 223 p.
[4] Astakhova VS. Stability of stromal precursor cells of human bone marrow preserved under various conditions. Gematol Transfuziol. 1987;32(8):40-3.
[5] Kulagina NN, Luriia EA, Astakhova VS, Genkina EN. Method of cloning human bone marrow stromal cells. Probl Gematol Pereliv Krovi. 1981;26(11):39-41.
[6] Astakhova VS. Comparative evaluation of xenofeeders in cloning of stromal fibroblasts of human bone marrow. Biull Eksp Biol Med. 1982;94(10):111-3.
[7] Astakhova VS. Cloning stromal fibroblasts of human bone marrow using heterologous feeder. Stem cell and tumor growth: Sat scientific. works. Kiev: Naukova Dumka, 1985: 120-123.
[8] Astakhova VS, Taran EV, Panchenko LM. Heterogeneity of the line of human bone marrow stromal precursor cells. Biopolym Cell. 1992; 8(4):43-8.
[9] Chailakhian RK, Gerasimov IuV, Fridenshteĭn AIa. Bone marrow microenvironment transfer by clones of stromal mechanocytes. Biull Eksp Biol Med. 1978;86(12):705-7. Russian.
[10] Astakhova VS, Nastenko EP, Oksamitna OG. Cloning indices and architectonics of human bone marrow stromal fibroblast colonies in diseases of various genesis. Biopolym Cell. 1994; 10(2):11-16.
[11] Mori KJ, Izumi H, Seto A. Stimulation and support of haemopoietic stem cell proliferation by irradiated stroma cell colonies in bone marrow cell culture in vitro. J Radiat Res. 1981;22(1):109-15.
[12] Gorskaia IuF, Fridenshteĭn AIa, Golovanova TA. Effects of growth factors on growth of stromal CFU-f in mouse bone marrow cell cultures. Biull Eksp Biol Med. 1991;112(12):615-7.