Biopolym. Cell. 1994; 10(6):88-91.
Individual isoacceptor tyrosine trnas from bovine liver. isolation and characteristic
1Kalachnyuk L. G.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Combination of two chromatographies of BD-cellulose in the presence and in the absence of Mg2+ was applied for high yield isolation of individual isoacceptor tyrosine tRNAs from bovine liver. The final purifacation of tyrosine tRNA was achieved by gel electrophoresis on a 7 % gel at 7 M urea. The nucleotide sequence of tRNATyr1 from bovine liver was determined to be identic to primaty structure of tyrosine tRNA Q·ψA from bovine liver (13) ( minor bases was not determined).


[1] Kovalenko MI, Zheltovskaya NI, El'skaya AV et al. Methods for isolation of total tRNA and individual tRNALeu and tRNAPhe from animal tissues. Methods of molecular biology. Kiev Naukova dumka, 1979; 98-111.
[2] Brunngraber EF. A simplified procedure for the preparation of "soluble" RNA from rat liver. Biochem Biophys Res Commun. 1962;8:1-3.
[3] Keller EB, Zamecnik PC. The effect of guanosine diphosphate and triphosphate on the incorporation of labeled amino acids into proteins. J Biol Chem. 1956;221(1):45-59.
[4] Kalachnyuk LG, Kozak LA, Tukalo MA, Matsuka GKh. Isolation and characteristic of the individual isoacceptor tRNA1Ser preparation from the bovine liver. Biopolym Cell. 1987; 3(5):274-276.
[5] Kalachnyuk LG, Tukalo MA, Matsuka GKh. Identification of phosphate residues involved in the formation of tertiary structure of tRNA1Ser from bovine liver. Biopolym Cell. 1992; 8(5):12-5.
[6] Peattie DA. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979;76(4):1760-4.
[7] Donis-Keller H, Maxam AM, Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977;4(8):2527-38.
[8] Garcia CM, Singhal RP. Affinity chromatography of tyrosine transfer RNAs. Biochem Biophys Res Commun. 1979;86(3):697-703.
[9] Wosnick MA, White BN. Purification and nucleoside composition of a Q* - containing aspartic acid tRNA from Drosophila. Biochem Biophys Res Commun. 1978;81(4):1131-8.
[10] Wosnick MA, White BN. The purification of Q-containing tRNAs by periodate modification. Purification and nucleoside composition of two related Drosophila tyrosine tRNAs. Biochim Biophys Acta. 1979;561(1):194-205.
[11] Sprinzl M, Hartmann T, Meissner F, Moll J, VorderwГјlbecke T. Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res. 1987;15 Suppl:r53-188.
[12] Johnson GD, Pirtle IL, Pirtle RM. The nucleotide sequence of tyrosine tRNAQ* psi A from bovine liver. Arch Biochem Biophys. 1985;236(1):448-53.
[13] Kisselev LL., Favorova OO, Lavrik OI. Biosynthesis of proteins from amino acids to aminoacyl-tRNA. Moscow, Nauka, 1984; 408 p.