Biopolym. Cell. 1994; 10(6):98-105.
The influence of the implanted cloning human's ApoAI gene on the development of rabbit' hypercholesterinemy
1Frolkis V. V., 2Kordyum V. A., 2Novikova S. N., 1Bogatskaya L. N., 2Irodov D. M., 2Likhacheva L. I., 2Potapenko R. I., 1Mozguhina T. G., 1Bitner M. K.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Gerontology Institute of the Academy of Medical Sciences of Ukraine
    67, Vyshgorodska Str., Kyiv, Ukraine, 04114


The effect of the implanted cloning human's apoAl gene on the dynamic of rabbit's lipid and lipoproteid metabolism in conditions of development of experimental hypercholesterinemy was investigated. The implantation of apoAl gene results in the appearance of human apolipoprotein Al in the blood of experimental animals, the changes in the content of cholesterine and it's shifts in different fractions of LP, the changes in the ratio of different classes of LP in favour Chs-HDLP and HDLP. It is supposed that transfection of apoAl human's gene and it's transitory expression leads not only to the biosynthesis of respective protein but also to a change in the work of total genome via gene-regulatory mechanisms.


[1] Gotto AM, Pwnall HJ. Manual of lipid disorders. New York : Williams and Wilkins, 1992: 125-161.
[2] Repin BS. Modern molecular cellular bases lipoprotein theory of atherosclerosis. Moscow: VNIIMI, 1987; 68 p.
[3] Frolkis VV. Genes-regulatory mechanisms of aging-base of age-related pathology. Fiziol Zh. 1990; 36(5) 3-11.
[4] High-density lipoproteins and atherosclerosis. Ed. AN Klimova, RI Levy. M.: Meditsine, 1983. 318 p.
[5] Sing ChF, Boerwinkle E, Moll PP. Apolipoproteins and cardiovascular risk: genetics and epidemiology. Davignon Annu Biol Clin. 1985;43: 411-7.
[6] Shulhzenko VN, Novikova SN, Kostetsky IE, Bogatskaya LN, Frolhis VV, Kordium VA. Transfer of cloned human gene ApoA1 into adult and old rabbits. Biopolym Cell. 1990; 6(2):71-6.
[7] Frolkis VV, Bogatskaya LN, Novikova SN, Muradian KK. Effects of protein biosynthesis inhibitors on lifespan and development of experimental atherosclerosis. Modification of the rate aging. Eds A. Ruiz-Torres, G. Hofecker. Wien. 1992;69-77.
[8] Gaal ?, Medgyesi G A, Vereczkey L. Electrophoresis in the separation of biological macromolecules. John Wiley & Sons, Chichester, UK and Akademiai Kiado, Budapest. 1980; 422 p.
[9] Lindgren FG, Gensen LC, Hatch FT. The isolation and analysis of serum lipoproteins. Blood lipids and lipoproteins: Quantitation, composition and metabolism. Ed. G. J. Nelson. New York: J. Wiley and Sons, 1990: 181-274.
[10] Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978;87(1):206-10.
[11] Kates M. Techniques of lipidology: isolation, analysis and identification of lipids. North-Holland Publishing Company, 1975; 269 p.
[12] Svanborg A, Svennerholm L, Myrén I, Soomägi MR, Claesson B, Rohman S. Plasma total lipid, cholesterol, triglycerides, phospholipids and free fatty acids in a healthy scandinavian population. Acta Med Scand. 1961;169(1):43–9.
[13] Swaney JB, Reese H, Eder HA. Polypeptide composition of rat high density lipoprotein: characterization by SDS-gel electrophoresis. Biochem Biophys Res Commun. 1974;59(2):513-9.
[14] Chaialo PP. Lipoprotein metabolism disorders. Kiev: Zdorov'ye, 1990. 182 p.
[15] Perov NV, Usatenko MS Composition apoproteins blood plasma at disalphalipoproteidemia. High-density lipoproteins and atherosclerosis. M .: Meditsina, 1983: 200-12.