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Thermodynamics of phenantridinium dye ethidium bromide interaction with deoxytetranucleotide 5'-d(ApCpGpT)
1Veselkov A. N., 1Djimant L. N., 1Bolotin P. A., 1Baranovsky S. F., 2Davies D.
  1. Sevastopol National Technical University
    33, Universytetska Str., Sevastopol, Ukraine, 99053
  2. Birkbeck, University of London
    Malet Str., Bloomsbury, London WC1E 7HX, UK


Temperature dependences of the chemical shifts of non-exchangeable ethidium bromide protons in a mixture with the deoxytetranucleoside triphosphate 5'-d(ApCpGpT) in aqueous solution have been investigated with a pulsed n. m. r. spectrometer (500 MHz). Original methods of calculation of thermodynamic parameters of complexing, which gives an opportunity to get individual contributions of each reaction leading to the formation of 1:1, 1:2, 2:1 and 2:2 complexes of dye and tetranucleotide molecules in conditions of the composite equilibrium in solution, have been used. The free Qibbs energies, enthalpies and entropies of these reactions have been determined.


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