Biopolym. Cell. 1995; 11(5):46-50.
Actinomycin D-induced oscillations in the intensity of nucleic acids synthesis in leukemia cells P-388
1Danko I. M., 2Prima V. I.
  1. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The effect of actinomycin D on the intensity of nucleic acids synthesis has been studied on leukemia cells P-388. It was found, that not only changes in degree of inhibition of RNA synthesis rate are related to the antibiotic concentration, but also kinetics of the process itself. Certain actinomycin D doses are found to cause cyclical variations in the DNA and RNA synthesis rate. But inhibition and activation periods alternating during the DNA and RNA synthesis coincide in time only partially and inhibition phase for RNA is two times the corresponding phase for DNA. Changes detected seem to have direct relationship with transcription regulation mechanisms.


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