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Organ-specific gene expression in transgenic potato: the cloning a new promoter of a class I patatin gene
1Yefimenko I. M., 1Medvedeva T. V., 1Kovalenko P. G., 2Gazaryan K. G., 1Galkin A. P.
  1. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094
  2. Institute of Molecular Genetics, Academy of Sciences of the USSR
    2, Academician Kurchatov Sq., Moscow, Russian Federation, 123182


Using synthetic oligonucleotide probes homologous to conservative AT-rich motif of patatin genes class I of two different clones were isolated from a potato genomic library. One of two different genomic clones named λpat122 was subcloned and analysis 5'-region sequences. Using the chloramphenicol acetyltransferase (CAT) gene as a reporter it has been shown that a 1.8 kb promoter fragment of the class I patatin gene λpat122 provides all the information necessary for both tuber-specific and sucrose-induced expression in leaves in transgenic potato plants.


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