Biopolym. Cell. 1996; 12(1):89-94.
Possible role of individual protein-DNA contacts of Flp site-specific recombinase from Saccharomyces cerevisiae with nucleic bases of Flp-binding element of DNA
1Voziyanov Y. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Site-specific recombinase Flp encoded by 2 micron plasmidfrom S. cerevisiae has multiple contacts with nucleic bases of Ftp-binding element of DNA. We present an analysis of relative contribution of these contacts to strength of Ftp-protein binding to DNA, and of the peculiarities of their distribution at major and minor grooves of DNA. The contacts of Flp-protein with Ftp-binding element of DNA have been divided into three groups depending on their contribution in strength of Flp-protein binding to DNA. It has been suggested that Flp-protein uses the third group of contacts (they make the least contribution to strength of Flp-protein binding to DNA) along with minor groove contacts for initial discrimination between different DNA sequences. It has been shown that Flp-protein has contacts with nucleic bases in minor groove at opposite sides of DNA, so Flp-protein probably wraps DNA.


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