Biopolym. Cell. 1996; 12(2):47-55.
Cell Biology
Kanamycin resistance of tobacco plants influenced by the preparations of DNAs on seeds
1Katsan V. A., 1Potopalsky A. I., 1Les'kiv M. E.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The direct influence of the exogenic pCAMVNEO DNA (cDNA) has been investigated on kanamycin resistance of the tobacco plants cultivar Krupnolystny 20 in the first and second generations ( T1 and T2). The pCAMVNEO DNA had been effected on the seeds in the aqueous solutions being applied separately as well as in a mixture with cDNAs originated from pTi8628 and the salt-tolerant black niglishade (Solatium nigrum). Kanamycin-resistant plants were obtained in considerable amount in all variants of experiment. The progeny of the resistant in T1 plants proved to be kanamycin-sensitive in T2. The correlation between the kanamycin resistance and the content of fundamental photosynthetic pigments was notrevealed among the most variants of the experiment. The possible kanamycin resistance mechanisms of tobacco in T1 are being discussed.


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