Biopolym. Cell. 1996; 12(5):38-45.
The role of regulatory arginyl-binding site of plasmine catalytic chain in fibrinogen hydrolysis
1Zolotaryova E. N., 1Bugrimenko N. N.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601


The effect of arginine and fibrinogen Dh-fragment on fibrinogenolysis velocity under the action of Lys77-plasmine and its partially degraded forms was studied. The results obtained revealed the low affinity to arginine of so-called arginyl-binding sites of the catalytic chain compared with arginyl-binding sites of the enzyme non-catalytic chain. At the same time fibrinogen hydrolysis inhibition at Dh-fragment presence was shown to be determined by the site located in the catalytic chain of Lys77- or Lys530-plasmine. This effect was found to occur at the stage of both X-fragments formation and their destruction. The role of catalytic chain regulatory site at some stages of fibrinogenolysis is discussed.


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